Diversity identification of KPC- producing Klebsiella pneumoniae using multilocus variable number tandem repeat analysis.

Mohammadi Bandari, N ; Keyvani, H ; et al.

In: Indian journal of medical microbiology, Jg. 40 (2022-04-01), Heft 2, S. 245-249

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Purpose: The current study aimed to determine blaKPC, blaGES, blaVIM, blaNDM, blaOXA-23, and blaOXA-48 genes in clinical strains of Klebsiella pneumoniae isolated in Tehran, Iran to assess genetic diversity using MLVA as a typing method.

Methods: A total of 181 K. pneumoniae isolates were obtained from various clinical samples. CLSI 2018 (clinical and laboratory standards institute) guidelines were used to determine antibiotic susceptibility and the Modified Hodge Test (MHT). To detect blaKPC, blaGES, blaVIM, blaNDM, blaOXA-23, and blaOXA-48, the polymerase chain reaction (PCR) method was used. The MLVA method was used to type K. pneumoniae isolates by using PCR for 8 Variable Number Tandem Repeats (VNTRs).

Results: Imipenem was the most effective antibiotic against K. pneumoniae, with 36.5% susceptibility. 100 (55.24%) of the isolates tested positive for KPC, and 30 (30%) tested positive for six genes. Thirty MLVA genotypes were distinguished, and an examination of diversity indexes (DIs) for eight loci revealed that seven different alleles were the most polymorphic, with the highest DI of 0.780.

Conclusions: The present study showed that MLVA could be helpful for typing clinical strains of K. pneumoniae. Our K. pneumoniae isolates are thought to be derived from a small number of clones that have undergone minor genetic changes over time. The results also showed that this method had great potential to differentiate those strains with high phenotypic similarity. The current study has revealed some intriguing facts about K. pneumoniae genetic relatedness in Tehran, Iran.

Competing Interests: Declaration of competing interest There are no conflicts to interest.

Titel:	Diversity identification of KPC- producing Klebsiella pneumoniae using multilocus variable number tandem repeat analysis.
Autor/in / Beteiligte Person:	Mohammadi Bandari, N ; Keyvani, H ; Abootaleb, M
Link:	Full Text Finder (Volltext)
Zeitschrift:	Indian journal of medical microbiology, Jg. 40 (2022-04-01), Heft 2, S. 245-249
Veröffentlichung:	2021- : [New York] : Elsevier ; <i>Original Publication</i>: New Delhi : Indian Association of Medical Microbiologists, 2022
Medientyp:	academicJournal
ISSN:	1998-3646 (electronic)
DOI:	10.1016/j.ijmmb.2022.01.003
Schlagwort:	Anti-Bacterial Agents pharmacology Bacterial Proteins genetics Humans Iran Microbial Sensitivity Tests Minisatellite Repeats Multilocus Sequence Typing methods beta-Lactamases genetics beta-Lactamases metabolism Klebsiella Infections microbiology Klebsiella pneumoniae genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Indian J Med Microbiol] 2022 Apr-Jun; Vol. 40 (2), pp. 245-249. <i>Date of Electronic Publication: </i>2022 Jan 21. MeSH Terms: Klebsiella Infections* / microbiology ; Klebsiella pneumoniae* / genetics ; Anti-Bacterial Agents / pharmacology ; Bacterial Proteins / genetics ; Humans ; Iran ; Microbial Sensitivity Tests ; Minisatellite Repeats ; Multilocus Sequence Typing / methods ; beta-Lactamases / genetics ; beta-Lactamases / metabolism Contributed Indexing: Keywords: Bacterial typing; Carbapenemase; Klebsiella pneumoniae; MLVA; VNTR Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (Bacterial Proteins) ; EC 3.5.2.6 (beta-Lactamases) Entry Date(s): Date Created: 20220125 Date Completed: 20220517 Latest Revision: 20220620 Update Code: 20240513

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