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Insertion/deletion markers (InDels) become an important marker for forensic medicine because of their compatible typing techniques with STRs and lower mutation rates. Recent years, a new kind of DNA marker named Multi-InDel was reported as characterized by two or more tightly linked InDel loci within a short length of physical position, usually 200-300 nucleotides. Many pieces of research showed that Multi-InDels had excellent application values in ancestry inference and forensic medicine. Since the identical number of insertion/deletion nucleotides of the InDel markers that composing the Multi-InDel marker, the genotypes of most reported Multi-InDels could not be directly typed by capillary electrophoresis (CE) due to the lack of length discrepancy among the composing InDel sequence. In this study, we applied a typing system of 20 Multi-InDels including 41 InDels, whose genotypes could be deduced by CE and assessed their potential applications in forensic medicine. A total of 200 unrelated Chinese Han individuals and five mother-child-father trios with proven paternity with one STR locus transmission incompatibilities from Shanxi province were genotyped by the multiplex system. The results showed that a total of 70 specific alleles were observed, more than three alleles were observed in 19 loci and seven alleles were observed in one locus. The combined probability of exclusion and the combined power of discrimination were 0.992 and 0.99999999993, respectively. This study demonstrates their potential usefulness for individual identification and paternity tests. The development of Multi-InDels provided another genetic tool inherent in higher polymorphic and lower mutation rates.

Titel:	A new set of 20 Multi-InDel markers for forensic application.
Autor/in / Beteiligte Person:	Liu, J ; Zhang, X ; Li, W ; Gao, L ; Li, J ; Wang, J ; Liu, Z ; Liu, Y ; Yan, J ; Zhang, G
Link:	Full Text Finder (Volltext)
Zeitschrift:	Electrophoresis, Jg. 43 (2022-06-01), Heft 11, S. 1193-1202
Veröffentlichung:	<Oct. 2004->: Weinheim : Wiley-VCH ; <i>Original Publication</i>: [Weinheim, Germany] : Verlag Chemie, [1980-, 2022
Medientyp:	academicJournal
ISSN:	1522-2683 (electronic)
DOI:	10.1002/elps.202100361
Schlagwort:	Alleles China Gene Frequency Genetics, Population Humans Male Nucleotides Forensic Genetics methods Genetic Markers INDEL Mutation Paternity
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Electrophoresis] 2022 Jun; Vol. 43 (11), pp. 1193-1202. <i>Date of Electronic Publication: </i>2022 Apr 18. MeSH Terms: Forensic Genetics* / methods ; Genetic Markers* ; INDEL Mutation* ; Paternity* ; Alleles ; China ; Gene Frequency ; Genetics, Population ; Humans ; Male ; Nucleotides References: Alonso A, Barrio PA, Müller P, Köcher S, Berger B, Martin P, et al. Current state-of-art of STR sequencing in forensic genetics. Electrophoresis 2018;39:2655-68. ; Fu J, Cheng J, Wei C, Khan MdA, Jin Z, Fu J. Assessing 23 Y-STR loci mutation rates in Chinese Han father-son pairs from southwestern China. Mol Biol Rep. 2020;47:7755-60. ; Prieto-Fernández E, Baeta M, Núñez C, Zarrabeitia MT, Herrera RJ, Builes JJ, et al. Development of a new highly efficient 17 X-STR multiplex for forensic purposes. 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A new set of 20 Multi-InDel markers for forensic application.