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Gold nanoparticles (GNPs) have shown great potential in diagnostic and therapeutic applications in diseases, such as cancer. Despite GNP versatility, there is conflicting data regarding the toxicity of their overall functionalization chemistry for improved biocompatibility. This study aimed to determine the possible genotoxic effects of functionalized GNPs in Human hepatocellular carcinoma (HepG2) cells. GNPs were synthesized and biofunctionalized with seven common molecules used for biomedical applications. These ligands were bovine serum albumin (BSA), poly(sodium 4-styrene sulfonate) (PSSNA), trisodium citrate (citrate), mercaptoundecanoic acid (MUA), glutathione (GSH), polyvinylpyrrolidone (PVP), and polyethylene glycol (PEG). Before in vitro genotoxicity assessment, inductively coupled plasma mass spectrometry was used to determine GNP cellular internalization quantitatively, followed by cell-based assays; WST-1 to find IC 30 and ApoPercentage for apoptotic induction time-points. The effect of the GNPs on cell growth in real-time was determined by using xCELLigence, followed by a comet assay for genotoxicity determination. The HepG2 cells experienced genotoxicity for all GNP ligands; however, they were able to initiate repair mechanisms and recover DNA damage, except for two functionalization chemistries.

Titel:	A Comparison of the Genotoxic Effects of Gold Nanoparticles Functionalized with Seven Different Ligands in Cultured Human Hepatocellular Carcinoma Cells.
Autor/in / Beteiligte Person:	Mulder, D ; Taute, CJF ; van Wyk M ; Pretorius, PJ
Link:	Full Text Finder (Volltext)
Zeitschrift:	Nanomaterials (Basel, Switzerland), Jg. 12 (2022-03-29), Heft 7
Veröffentlichung:	Basel, Switzerland : MDPI AG, [2011]-, 2022
Medientyp:	academicJournal
ISSN:	2079-4991 (print)
DOI:	10.3390/nano12071126
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Nanomaterials (Basel)] 2022 Mar 29; Vol. 12 (7). <i>Date of Electronic Publication: </i>2022 Mar 29. References: J Toxicol Environ Health A. 1999 Aug 13;57(7):489-505. (PMID: 10494917) ; J Nanopart Res. 2016 Jun;18:159. (PMID: 27594804) ; J Mater Chem B. 2013 Feb 21;1(7):1010-1018. (PMID: 32262365) ; Biol Trace Elem Res. 2018 Jul;184(1):226-239. (PMID: 28994012) ; Mutat Res. 1997 Apr 29;375(2):183-93. (PMID: 9202728) ; Nanomedicine. 2013 Feb;9(2):257-63. (PMID: 22772047) ; FEBS Lett. 2012 Nov 2;586(21):3926-30. (PMID: 23044009) ; Carbohydr Polym. 2014 Jan 30;101:897-904. (PMID: 24299854) ; Toxicol In Vitro. 2021 Feb;70:105046. (PMID: 33147519) ; Cell Biol Toxicol. 2016 Aug;32(4):305-21. (PMID: 27184667) ; Curr Top Med Chem. 2015;15(16):1605-13. (PMID: 25877087) ; Aquat Toxicol. 2010 Oct 15;100(2):178-86. (PMID: 20382436) ; J Am Chem Soc. 2009 Apr 29;131(16):5728-9. (PMID: 19351115) ; Adv Drug Deliv Rev. 2010 Mar 8;62(3):346-61. (PMID: 19914317) ; Kidney Int. 2001 Jul;60(1):370-4. (PMID: 11422774) ; Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1173-1180. (PMID: 30942109) ; Biomed Pharmacother. 2003 May-Jun;57(3-4):145-55. (PMID: 12818476) ; Nanomedicine. 2012 Aug;8(6):804-14. (PMID: 22306155) ; Nanotoxicology. 2014 May;8(3):233-78. (PMID: 23379603) ; Br J Radiol. 2012 Feb;85(1010):101-13. (PMID: 22010024) ; Biomaterials. 2009 May;30(14):2790-8. (PMID: 19232712) ; Oxid Med Cell Longev. 2013;2013:956792. (PMID: 23738047) ; J Am Pharm Assoc Am Pharm Assoc. 1954 Aug;43(8):483-5. (PMID: 13191996) ; Nanomaterials (Basel). 2020 Feb 06;10(2):. (PMID: 32041143) ; Chem Rev. 2004 Jan;104(1):293-346. (PMID: 14719978) ; J Biol Chem. 1996 Dec 20;271(51):32994-3001. (PMID: 8955144) ; Nanomedicine. 2007 Jun;3(2):111-9. (PMID: 17572353) ; Int J Mol Sci. 2015 Mar 31;16(4):7210-29. (PMID: 25837469) ; Anticancer Res. 2011 Mar;31(3):797-805. (PMID: 21498699) ; Nanomedicine. 2012 Feb;8(2):147-66. (PMID: 21703993) ; J Toxicol Environ Health B Crit Rev. 2020 May 18;23(4):137-176. (PMID: 32321383) ; Exp Cell Res. 1988 Mar;175(1):184-91. (PMID: 3345800) ; Polymers (Basel). 2020 Nov 08;12(11):. (PMID: 33171660) ; Langmuir. 2012 Apr 24;28(16):6618-31. (PMID: 22471950) ; Nanotechnology. 2008 Jul 23;19(29):295104. (PMID: 21730596) ; Anal Chem. 2007 Jun 1;79(11):4215-21. (PMID: 17458937) ; Crit Care Med. 1989 Jul;17(7):690-4. (PMID: 2661149) ; Biometals. 2012 Oct;25(5):1009-22. (PMID: 22752843) ; Biochem Biophys Res Commun. 2005 Dec 16;338(2):815-9. (PMID: 16263080) ; Biochem Biophys Rep. 2021 Apr 10;26:100991. (PMID: 33912692) ; Crit Care. 2012 Dec 07;16(6):249. (PMID: 23216871) ; Int J Nanomedicine. 2019 Nov 07;14:8787-8804. (PMID: 31806972) ; Nanotoxicology. 2018 Apr;12(3):251-262. (PMID: 29392969) ; J Appl Toxicol. 2013 Oct;33(10):1111-9. (PMID: 23529830) ; Int J Mol Sci. 2021 Dec 04;22(23):. (PMID: 34884942) Contributed Indexing: Keywords: HepG2; biofunctionalization; comet assay; cytotoxicity; genotoxicity; gold nanoparticles Entry Date(s): Date Created: 20220412 Latest Revision: 20220423 Update Code: 20240513 PubMed Central ID: PMC9000686

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A Comparison of the Genotoxic Effects of Gold Nanoparticles Functionalized with Seven Different Ligands in Cultured Human Hepatocellular Carcinoma Cells.