Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae .
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 120 (2023-03-21), Heft 12, S. e2301358120
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Zugriff:
To cause rice blast disease, the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during plant infection and show very low expression during other developmental stages. How effector gene expression is regulated in such a precise manner during invasive growth by M. oryzae is not known. Here, we report a forward-genetic screen to identify regulators of effector gene expression, based on the selection of mutants that show constitutive effector gene expression. Using this simple screen, we identify Rgs1, a regulator of G-protein signaling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. We show that an N-terminal domain of Rgs1, possessing transactivation activity, is required for effector gene regulation and acts in an RGS-independent manner. Rgs1 controls the expression of at least 60 temporally coregulated effector genes, preventing their transcription during the prepenetration stage of development prior to plant infection. A regulator of appressorium morphogenesis is therefore also required for the orchestration of pathogen gene expression required for invasive growth by M. oryzae during plant infection.
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Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae .
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Autor/in / Beteiligte Person: | Tang, B ; Yan, X ; Ryder, LS ; Bautista, MJA ; Cruz-Mireles, N ; Soanes, DM ; Molinari, C ; Foster, AJ ; Talbot, NJ |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 120 (2023-03-21), Heft 12, S. e2301358120 |
Veröffentlichung: | Washington, DC : National Academy of Sciences, 2023 |
Medientyp: | academicJournal |
ISSN: | 1091-6490 (electronic) |
DOI: | 10.1073/pnas.2301358120 |
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