A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.
In: Cell reports methods, Jg. 3 (2023-03-31), Heft 4, S. 100441
Online
academicJournal
Zugriff:
Hyperspectral fluorescence imaging improves multiplexed observations of biological samples by utilizing multiple color channels across the spectral range to compensate for spectral overlap between labels. Typically, spectral resolution comes at a cost of decreased detection efficiency, which both hampers imaging speed and increases photo-toxicity to the samples. Here, we present a high-speed, high-efficiency snapshot spectral acquisition method, based on optical compression of the fluorescence spectra via Fourier transform, that overcomes the challenges of discrete spectral sampling: single-shot hyperspectral phasor camera (SHy-Cam). SHy-Cam captures fluorescence spatial and spectral information in a single exposure with a standard scientific CMOS camera, with photon efficiency of over 80%, easily and with acquisition rates exceeding 30 datasets per second, making it a powerful tool for multi-color in vivo imaging. Its simple design, using readily available optical components, and its easy integration provide a low-cost solution for multi-color fluorescence imaging with increased efficiency and speed.
Competing Interests: The University of Southern California has filed a provisional patent application covering this method listing P.W., S.E.F., and F.C. as inventors.
(© 2023 The Authors.)
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A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.
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Autor/in / Beteiligte Person: | Wang, P ; Kitano, M ; Keomanee-Dizon, K ; Truong, TV ; Fraser, SE ; Cutrale, F |
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Zeitschrift: | Cell reports methods, Jg. 3 (2023-03-31), Heft 4, S. 100441 |
Veröffentlichung: | [New York] : Elsevier Inc., [2021]-, 2023 |
Medientyp: | academicJournal |
ISSN: | 2667-2375 (electronic) |
DOI: | 10.1016/j.crmeth.2023.100441 |
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