Einzeltreffer — DigiBib

Acrosome is inextricably related to membranous organelles. The origin of acrosome is still controversial, one reason is that limited articles were reported about the proteomic analysis of the acrosome. Mitochondrial proteins were found exist in the acrosome, nevertheless, only limited attention has been paid to the function of mitochondrial proteins in the acrosome formation. Eriocheir sinensis sperm has a large acrosome, which makes it an ideal model to study acrosome formation. Here, we firstly compared the rate of acrosome reaction induced by the calcium ionophore A23187 and ionomycin. The rate of acrosome reaction induced by ionomycin is higher (95.8%) than A23187 (58.7%). Morphological changes were observed using light, confocal and transmission electron microscopy. Further more, proteins released during the acrosome reaction as induced by ionomycin were collected for LC-MS/MS analysis. A total of 945 proteins, including malate dehydrogenase (MDH) and voltage-dependent anion channel 3 (VDAC3), were identified in the acrosomal released proteome. The number of proteins from mitochondria (17.57%) was higher compared with endoplasmic reituculum (1.59%) and lysosomes (1.8%). To investigate the functions of target mitochondrial proteins during spermatogenesis, poly-antibodies of MDH in E. sinensis were prepared. The characteristics, further analyzed using immunofluorescence, of two mitochondrial proteins during acrosome formation showed that MDH and VDAC3 were independently involved in the formation of acrosomal membrane. These findings illustrate the acrosomal released proteome and provide important data resource for understanding the relationship between mitochondria and the acrosome in Decapoda crustacean.

Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Titel:	Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis.
Autor/in / Beteiligte Person:	Li, C ; Yu, R ; Liu, H ; Qiao, J ; Zhang, F ; Mu, S ; Guo, M ; Zhang, H ; Li, Y ; Kang, X
Zeitschrift:	Gene, Jg. 887 (2023-12-15), S. 147784
Veröffentlichung:	Amsterdam, Elsevier/North-Holland, 1976-, 2023
Medientyp:	academicJournal
ISSN:	1879-0038 (electronic)
DOI:	10.1016/j.gene.2023.147784
Schlagwort:	Male Humans Acrosome Calcimycin Chromatography, Liquid Ionomycin Proteomics Semen Tandem Mass Spectrometry Spermatozoa Spermatogenesis Mitochondria Mitochondrial Proteins Voltage-Dependent Anion Channels Lysosomes Proteome Malate Dehydrogenase
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Gene] 2023 Dec 15; Vol. 887, pp. 147784. <i>Date of Electronic Publication: </i>2023 Sep 07. MeSH Terms: Proteome* ; Malate Dehydrogenase* ; Male ; Humans ; Acrosome ; Calcimycin ; Chromatography, Liquid ; Ionomycin ; Proteomics ; Semen ; Tandem Mass Spectrometry ; Spermatozoa ; Spermatogenesis ; Mitochondria ; Mitochondrial Proteins ; Voltage-Dependent Anion Channels ; Lysosomes Contributed Indexing: Keywords: Acrosome formation; Acrosome reaction; Eriocheir sinensis; Mitochondria; Spermatogenesis Substance Nomenclature: 0 (Proteome) ; EC 1.1.1.37 (Malate Dehydrogenase) ; 37H9VM9WZL (Calcimycin) ; 56092-81-0 (Ionomycin) ; 0 (Mitochondrial Proteins) ; 0 (Voltage-Dependent Anion Channels) Entry Date(s): Date Created: 20230909 Date Completed: 20230925 Latest Revision: 20230925 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis.