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More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus epidemics. Virus detection in seed stocks remains the most frequent approach for seed health testing, but current methods are not always standardized and/or do not allow analyzing large numbers of seeds. Here, we describe a high-throughput method to quantify plant virus seed transmission rate based on classical grow-out tests, which can be applied to widely different viruses and host species.

Titel:	Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana.
Autor/in / Beteiligte Person:	Gil-Valle, M ; Sáez, C ; Montes, N ; Pagán, I
Zeitschrift:	Methods in molecular biology (Clifton, N.J.), Jg. 2724 (2024), S. 181-192
Veröffentlichung:	Totowa, NJ : Humana Press ; <i>Original Publication</i>: Clifton, N.J. : Humana Press,, 2024
Medientyp:	academicJournal
ISSN:	1940-6029 (electronic)
DOI:	10.1007/978-1-0716-3485-1_13
Schlagwort:	Seeds Host Specificity Arabidopsis Plant Viruses genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Methods Mol Biol] 2024; Vol. 2724, pp. 181-192. MeSH Terms: Arabidopsis* ; Plant Viruses* / genetics ; Seeds ; Host Specificity References: Sastry KS (2013) Seed-borne plant virus diseases. Springer, New Delhi. (PMID: 10.1007/978-81-322-0813-6) ; Kumar PL, Cuervo M, Kreuze JF et al (2021) Phytosanitary interventions for safe global germplasm exchange and the prevention of transboundary pest spread: the role of CGIAR germplasm health units. Plants 10:328. ; Verhoeven A, Kloth KJ, Kupczok A et al (2023) Arabidopsis latent virus 1, a comovirus widely spread in Arabidopsis thaliana collections. New Phytol 237:1146–1153. (PMID: 10.1111/nph.18466) ; Chalam VC (2020) Elimination of plant viruses by certification and quarantine for ensuring biosecurity. In: Awasthi LP (ed) Applied plant virology. Advances, detection, and antiviral strategies, 1st edn. Academic, Cambridge, pp 749–762. (PMID: 10.1016/B978-0-12-818654-1.00052-9) ; Pagán I (2019) Movement between plants: vertical transmission. In: Palukaitis P, García-Arenal F (eds) Cucumber mosaic virus. APS Press, St. Paul, pp 185–198. (PMID: 10.1094/9780890546109.020) ; Pagán I (2022) Transmission through seeds: the unknown life of plant viruses. PLoS Pathog 18:e1010707. (PMID: 10.1371/journal.ppat.1010707) ; Salem NM, Ehlers JD, Roberts PA et al (2010) Biological and molecular diagnosis of seedborne viruses in cowpea germplasm of geographically diverse sub-Saharan origins. Plant Pathol 59:773–784. (PMID: 10.1111/j.1365-3059.2010.02285.x) ; Pina MAS, Gómez-Aix C, Méndez-López E et al (2021) Imaging techniques to study plant virus replication and vertical transmission. Viruses 13:358. (PMID: 10.3390/v13030358) ; Torre C, Agüero J, Gómez-Aix C et al (2020) Comparison of DAS-ELISA and qRT-PCR for the detection of cucurbit viruses in seeds. Ann Appl Biol 176:158–169. (PMID: 10.1111/aab.12543) ; Kaur SI, Kashyap PL, Kang SS et al (2020) Detection and diagnosis of seed-borne viruses and virus-like pathogens. In: Kumar R, Gupta A (eds) Seed-borne diseases of agricultural crops: detection, diagnosis & management. Springer, Singapore, pp 169–199. (PMID: 10.1007/978-981-32-9046-4_7) ; Ellis MD, Hoak JM, Ellis BW et al (2020) Quantitative real-time PCR analysis of individual flue-cured tobacco seeds and seedlings reveals seed transmission of Tobacco mosaic virus. Phytopathology 110:194–205. ; Davino S, Caruso AG, Bertacca S et al (2020) Tomato brown rugose fruit virus: seed transmission rate and efficacy of different seed disinfection treatments. Plants 9:1615. ; Bester R, Cook G, Breytenbach JH et al (2021) Towards the validation of high-throughput sequencing (HTS) for routine plant virus diagnostics: measurement of variation linked to HTS detection of citrus viruses and viroids. Virol J 18:1–19. (PMID: 10.1186/s12985-021-01523-1) ; Vishunavat K, Prabakar K, Anand T (2023) Seed health: testing and management. In: Dadlani M, Yadava DK (eds) Seed science and technology. Springer, Singapore, pp 335–364. (PMID: 10.1007/978-981-19-5888-5_14) ; International Seed Testing Association (ISTA) (2023) International rules for seed testing. https://www.seedtest.org/en/useful-links-footer/ista-rules.html . Accessed on 29 April 2023. ; Pagán I, Alonso-Blanco C, García-Arenal F (2007) The relationship of within-host multiplication and virulence in a plant-virus system. PLoS One 2:e786. (PMID: 10.1371/journal.pone.0000786) ; Montes N, Alonso-Blanco C, García-Arenal F (2019) Cucumber mosaic virus infection as a potential selective pressure on Arabidopsis thaliana populations. PLoS Pathog 15:e1007810. (PMID: 10.1371/journal.ppat.1007810) ; Cobos A, Montes N, López-Herranz M et al (2019) Within-host multiplication and speed of colonization as infection traits associated with plant virus vertical transmission. J Virol 93:e01078–e01019. (PMID: 10.1128/JVI.01078-19) ; Gibbs AJ, Gower JC (1960) The use of a multi-transfer method in plant virus transmission studies: some statistical points arising in the analysis of results. Ann Appl Biol 48:75–83. (PMID: 10.1111/j.1744-7348.1960.tb03506.x) ; Zuzarte PC, Denroche RE, Fehringer G et al (2014) A two-dimensional pooling strategy for rare variant detection on next- generation sequencing platforms. PLoS One 9:e93455. (PMID: 10.1371/journal.pone.0093455) ; Montes N, Cobos A, Gil-Valle M et al (2021) Arabidopsis thaliana genes associated with Cucumber mosaic virus virulence and their link to virus seed transmission. Microorganisms 9:692. Contributed Indexing: Keywords: Arabidopsis thaliana; Grow-out test; Plant virus; RT-qPCR virus detection; Seed transmission Entry Date(s): Date Created: 20231121 Date Completed: 20231127 Latest Revision: 20231127 Update Code: 20240514

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Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana.