Einzeltreffer — DigiBib

Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC 3D-hIO ) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC 3D-hIO , combined with air-liquid interface culture in a minimally defined medium, forces ISC 3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC 3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC 3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC 3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling.

Titel:	Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids.
Autor/in / Beteiligte Person:	Kwon, O ; Lee, H ; Jung, J ; Son, YS ; Jeon, S ; Yoo, WD ; Son, N ; Jung, KB ; Choi, E ; Lee, IC ; Kwon, HJ ; Kim, C ; Lee, MO ; Cho, HS ; Kim, DS ; Son, MY
Link:	View record at Springer (Volltext)
Zeitschrift:	Nature communications, Jg. 15 (2024-01-27), Heft 1, S. 799
Veröffentlichung:	[London] : Nature Pub. Group, 2024
Medientyp:	academicJournal
ISSN:	2041-1723 (electronic)
DOI:	10.1038/s41467-024-45103-7
Schlagwort:	Humans Reproducibility of Results Intestinal Mucosa Organoids Cell Differentiation Intestines Pluripotent Stem Cells
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Nat Commun] 2024 Jan 27; Vol. 15 (1), pp. 799. <i>Date of Electronic Publication: </i>2024 Jan 27. MeSH Terms: Intestines* ; Pluripotent Stem Cells* ; Humans ; Reproducibility of Results ; Intestinal Mucosa ; Organoids ; Cell Differentiation References: Nat Med. 2011 Sep 04;17(10):1225-7. (PMID: 21892181) ; Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168) ; Adv Drug Deliv Rev. 2014 Apr;69-70:19-28. (PMID: 24582599) ; Cell Stem Cell. 2018 Feb 1;22(2):171-176.e5. (PMID: 29290616) ; Gastroenterology. 2011 Nov;141(5):1762-72. (PMID: 21889923) ; Front Cell Dev Biol. 2020 Oct 23;8:593969. (PMID: 33195268) ; Am J Physiol Lung Cell Mol Physiol. 2020 Jul 1;319(1):L39-L44. (PMID: 32491949) ; Development. 2017 Mar 15;144(6):958-962. (PMID: 28292841) ; Dev Cell. 2020 Dec 21;55(6):771-783.e5. (PMID: 33290721) ; Trends Mol Med. 2017 May;23(5):393-410. (PMID: 28341301) ; Science. 2020 Jul 3;369(6499):50-54. (PMID: 32358202) ; Nature. 2011 Feb 3;470(7332):105-9. (PMID: 21151107) ; Nature. 2015 Jun 11;522(7555):173-8. (PMID: 26040716) ; Nat Commun. 2018 Aug 2;9(1):3039. (PMID: 30072687) ; Sci Rep. 2019 Jul 12;9(1):10140. (PMID: 31300688) ; Nat Struct Mol Biol. 2019 May;26(5):372-379. (PMID: 31036956) ; Nat Cell Biol. 2016 Mar;18(3):246-54. (PMID: 26911908) ; Nat Biomed Eng. 2020 Sep;4(9):863-874. (PMID: 32514094) ; Nat Protoc. 2015 Oct;10(10):1474-85. (PMID: 26334867) ; Sci Adv. 2021 Jun 2;7(23):. (PMID: 34078609) ; Front Mol Biosci. 2021 Sep 21;8:708336. (PMID: 34631794) ; Proc Biol Sci. 2015 Dec 22;282(1821):20143085. (PMID: 26702035) ; Gastroenterology. 2013 Aug;145(2):383-95.e1-21. (PMID: 23644405) ; Cell. 2021 Jun 10;184(12):3281-3298.e22. (PMID: 34019796) ; Antiviral Res. 2021 Aug;192:105115. (PMID: 34157321) ; Nature. 2021 Sep;597(7875):250-255. (PMID: 34497389) ; Cell Stem Cell. 2013 Dec 5;13(6):734-44. (PMID: 24139758) ; J Clin Med. 2019 Jul 04;8(7):. (PMID: 31277507) ; Stem Cell Reports. 2015 Jun 3;:. (PMID: 26050928) ; J Tissue Eng. 2021 Feb 1;12:2041731420985202. (PMID: 34104387) ; Nature. 2017 May 11;545(7653):234-237. (PMID: 28467818) ; Nature. 2021 Apr;592(7852):99-104. (PMID: 33627870) ; Nat Commun. 2016 Jan 19;7:10380. (PMID: 26783136) ; Physiol Rev. 1999 Oct;79(4):1193-226. (PMID: 10508233) ; Dev Dyn. 2011 Mar;240(3):501-20. (PMID: 21246663) ; Nat Med. 2014 Nov;20(11):1310-4. (PMID: 25326803) ; Neuropathol Appl Neurobiol. 2017 Dec;43(7):584-603. (PMID: 28235153) ; Asian Pac J Allergy Immunol. 2020 Mar;38(1):1-9. (PMID: 32105090) ; Bioinformatics. 2007 Mar 15;23(6):657-63. (PMID: 17234643) ; Respir Res. 2020 Aug 27;21(1):224. (PMID: 32854739) ; Commun Biol. 2023 Apr 8;6(1):387. (PMID: 37031303) ; Front Immunol. 2020 Oct 07;11:576745. (PMID: 33117379) Grant Information: NRF-2018M3A9H3023077, 2021M3A9H3016046 Ministry of Science, ICT and Future Planning (MSIP); 20008777 Ministry of Trade, Industry and Energy (Ministry of Trade, Industry and Energy, Korea); 22213MFDS386 Ministry of Food and Drug Safety (MFDS); NBS7942211 National Research Foundation of Korea (NRF) Entry Date(s): Date Created: 20240127 Date Completed: 20240129 Latest Revision: 20240130 Update Code: 20240130 PubMed Central ID: PMC10821882

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Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids.