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Aim: Lithospermum erythrorhizon and Pueraria lobata exhibit promising potential as cosmetic additives for mitigating skin barrier impairment induced by photoaging. Despite their potential, the precise mechanisms underlying their protective and ameliorative effects remain elusive. This study sought to assess the reparative properties of Lithospermum erythrorhizon and Pueraria lobata extracts (LP) on UVB-irradiated human skin keratinocytes (HaCaT cells) and explore the therapeutic potential of LP as a skin barrier protection agent.

Materials and Methods: Antioxidant activities were gauged through 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reactive oxygen species (ROS) assays. The expression levels of skin barrier-related markers, encompassing metalloproteinases (MMPs) and hyaluronidase (HYAL) were scrutinized using enzyme-linked immunosorbent assay (ELISA), reverse transcriptase (RT)-PCR, and Western blotting, with a particular focus on the involvement of the transforming growth factor (TGF)-β/Smad and nuclear factor-κB (NF-κB) signaling pathways.

Results: The study revealed that LP effectively scavenges free radicals, diminishes ROS production in a dose-dependent manner, and significantly attenuates UVB-induced expression of MMP-1 and MMP-3 through modulation of the hyaluronan synthase (HAS)2/HYAL1 signaling axis in UVB-irradiated HaCaT cells. Additionally, LP demonstrated enhanced TGF-β signaling activation, fostering procollagen type I synthesis, and concurrently exhibited mitogen-activated protein kinases (MAPK)/NF-κB signaling inactivation, thereby mitigating pro-inflammatory cytokine release and alleviating UVB-induced cellular damage.

Conclusion: In conclusion, the observed protective effects of LP on skin cellular constituents highlight its substantial biological potential for shielding against UVB-induced skin photoaging, positioning it as a promising candidate for both pharmaceutical and cosmetic applications.

Titel:	Photoprotective effects of Lithospermum erythrorhizon and Pueraria lobata extracts on UVB-induced photoaging: A study on skin barrier protection.
Autor/in / Beteiligte Person:	Lee, S ; Oh, S ; Zheng, Q ; Zheng, S ; Kim, M ; Park, S ; Choi, W ; Yin, CS ; Yi, TH
Link:	Full Text Finder (Volltext)
Zeitschrift:	Photodermatology, photoimmunology & photomedicine, Jg. 40 (2024), Heft 1, S. e12950
Veröffentlichung:	<2010-> : Oxford : Blackwell ; <i>Original Publication</i>: Copenhagen : Munksgaard, c1990-, 2024
Medientyp:	academicJournal
ISSN:	1600-0781 (electronic)
DOI:	10.1111/phpp.12950
Schlagwort:	Humans NF-kappa B metabolism NF-kappa B pharmacology Reactive Oxygen Species metabolism Skin metabolism Ultraviolet Rays adverse effects Plant Extracts pharmacology Plant Extracts chemistry Fibroblasts metabolism Pueraria metabolism Lithospermum metabolism Skin Aging Skin Diseases
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Photodermatol Photoimmunol Photomed] 2024 Jan; Vol. 40 (1), pp. e12950. MeSH Terms: Pueraria* / metabolism ; Lithospermum* / metabolism ; Skin Aging* ; Skin Diseases* ; Humans ; NF-kappa B / metabolism ; NF-kappa B / pharmacology ; Reactive Oxygen Species / metabolism ; Skin / metabolism ; Ultraviolet Rays / adverse effects ; Plant Extracts / pharmacology ; Plant Extracts / chemistry ; Fibroblasts / metabolism References: Kohl E, Steinbauer J, Landthaler M, Szeimies RM. Skin ageing. J Eur Acad Dermatol Venereol. 2011;25(8):873-884. doi:10.1111/j.1468-3083.2010.03963.x. ; Kageyama H, Waditee-Sirisattha R. Antioxidative, anti-inflammatory, and anti-aging properties of Mycosporine-like amino acids: molecular and cellular mechanisms in the protection of skin-aging. Mar Drugs. 2019;17(4):222. doi:10.3390/md17040222. ; Miao-miao G, Wan-ting X, Lian-ying L, et al. 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Front Pharmacol. 2018;9:392. doi:10.3389/fphar.2018.00392. Contributed Indexing: Keywords: Lithospermum erythrorhizon; Pueraria lobata; photoaging; skin barrier; ultraviolet B (UVB) Substance Nomenclature: 0 (NF-kappa B) ; 0 (Reactive Oxygen Species) ; 0 (Plant Extracts) Entry Date(s): Date Created: 20240130 Date Completed: 20240131 Latest Revision: 20240131 Update Code: 20240131

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Photoprotective effects of Lithospermum erythrorhizon and Pueraria lobata extracts on UVB-induced photoaging: A study on skin barrier protection.