Einzeltreffer — DigiBib

Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.

Competing Interests: YL, LW, YC, YC, SH, SW, CC, GH, SY, SC, KW, CL, PH, HC, BC, LC No competing interests declared

Titel:	Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.
Autor/in / Beteiligte Person:	Lin, YH ; Wang, LW ; Chen, YH ; Chan, YC ; Hu, SH ; Wu, SY ; Chiang, CS ; Huang, GJ ; Yang, SD ; Chu, SW ; Wang, KC ; Lin, CH ; Huang, PH ; Cheng, HJ ; Chen, BC ; Chu, LA
Link:	Full Text Finder (Volltext)
Zeitschrift:	ELife, Jg. 13 (2024-05-22)
Veröffentlichung:	Cambridge, UK : eLife Sciences Publications, Ltd., 2012-, 2024
Medientyp:	academicJournal
ISSN:	2050-084X (electronic)
DOI:	10.7554/eLife.93212
Schlagwort:	Animals Mice Fixatives chemistry Paraffin Embedding methods Formaldehyde Brain Tissue Fixation methods Neurons physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Elife] 2024 May 22; Vol. 13. <i>Date of Electronic Publication: </i>2024 May 22. MeSH Terms: Paraffin Embedding* / methods ; Formaldehyde* ; Brain* ; Tissue Fixation* / methods ; Neurons* / physiology ; Animals ; Mice ; Fixatives / chemistry References: Nat Biotechnol. 2018 Dec 17;:. (PMID: 30556815) ; Behav Brain Res. 2018 Aug 1;348:115-126. (PMID: 29684476) ; Nature. 2013 May 16;497(7449):332-7. (PMID: 23575631) ; Cell. 2020 Feb 20;180(4):796-812.e19. (PMID: 32059778) ; Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) ; J Am Soc Mass Spectrom. 2020 Aug 5;31(8):1619-1624. (PMID: 32678590) ; J Pathol. 1997 Sep;183(1):116-23. (PMID: 9370957) ; J Pathol Inform. 2018 Dec 24;9:46. (PMID: 30662792) ; Nat Neurosci. 2015 Oct;18(10):1518-29. (PMID: 26368944) ; IEEE Trans Med Imaging. 2010 Jan;29(1):196-205. (PMID: 19923044) ; J R Coll Physicians Edinb. 2020 Dec;50(4):398-402. (PMID: 33469615) ; BMC Genomics. 2019 Mar 29;20(1):249. (PMID: 30922218) ; Nat Protoc. 2012 Nov;7(11):1983-95. (PMID: 23060243) ; Methods Mol Biol. 2023;2561:3-30. (PMID: 36399262) ; Brain. 2018 Mar 1;141(3):797-810. (PMID: 29360949) ; Nat Methods. 2020 Apr;17(4):442-449. (PMID: 32161395) ; Nat Neurosci. 2011 Aug 30;14(11):1481-8. (PMID: 21878933) ; Cell Rep. 2018 Aug 21;24(8):2196-2210.e9. (PMID: 30134179) ; Cell Rep Methods. 2023 Apr 20;3(5):100458. (PMID: 37323568) ; Sci Rep. 2019 Apr 4;9(1):5624. (PMID: 30948791) ; Nat Biotechnol. 2016 Sep;34(9):987-92. (PMID: 27376584) ; Nat Methods. 2007 Apr;4(4):331-6. (PMID: 17384643) ; Cell. 2015 Jul 16;162(2):246-257. (PMID: 26186186) ; iScience. 2020 Aug 21;23(8):101432. (PMID: 32805648) ; Can J Neurol Sci. 2015 Mar;42(2):74-87. (PMID: 25804247) ; J Digit Imaging. 2020 Aug;33(4):1034-1040. (PMID: 32468487) ; Cell. 2015 Dec 3;163(6):1500-14. (PMID: 26638076) ; Lab Invest. 2012 Jan;92(1):151-62. (PMID: 21894147) ; Biotech Histochem. 1996 Sep;71(5):263-70. (PMID: 8896801) ; Nucleic Acids Res. 2021 Dec 2;49(21):e125. (PMID: 34534335) ; J Pathol. 1994 Aug;173(4):371-9. (PMID: 7965396) ; Nat Biotechnol. 2017 Aug;35(8):757-764. (PMID: 28714966) ; Cell. 2014 Apr 24;157(3):726-39. (PMID: 24746791) ; Small Methods. 2022 Jan;6(1):e2100943. (PMID: 35041279) ; Nat Neurosci. 2019 Feb;22(2):317-327. (PMID: 30598527) ; Nat Protoc. 2021 Jan;16(1):239-262. (PMID: 33247285) ; J Histochem Cytochem. 2001 Aug;49(8):931-7. (PMID: 11457921) ; J Histochem Cytochem. 1991 Jun;39(6):741-8. (PMID: 1709656) ; Nat Rev Neurosci. 2020 Feb;21(2):61-79. (PMID: 31896771) ; HFSP J. 2008 Oct;2(5):266-75. (PMID: 19404438) ; Nat Commun. 2016 Jul 07;7:11879. (PMID: 27384127) ; Histochem Cell Biol. 2001 Aug;116(2):119-30. (PMID: 11685540) ; Cell Res. 2018 Aug;28(8):803-818. (PMID: 29844583) ; Histochem Cell Biol. 1996 Apr;105(4):261-7. (PMID: 9072183) ; Nat Biomed Eng. 2017 Oct;1(10):796-806. (PMID: 31015588) ; Front Aging Neurosci. 2015 Oct 31;7:196. (PMID: 26582988) ; J Pathol Inform. 2021 Dec 09;12:50. (PMID: 35070479) ; BMC Cancer. 2020 Apr 7;20(1):291. (PMID: 32264863) ; Methods Mol Biol. 2010;588:103-19. (PMID: 20012825) ; Nat Biotechnol. 2024 Apr;42(4):617-627. (PMID: 37430076) ; Front Neurol. 2020 Aug 20;11:833. (PMID: 32973652) ; Cell Rep. 2016 Jul 26;16(4):1138-1152. (PMID: 27425620) ; Sci Rep. 2017 Aug 24;7(1):9269. (PMID: 28839164) ; iScience. 2021 Feb 12;24(3):102178. (PMID: 33718830) ; Nat Commun. 2020 Apr 27;11(1):1982. (PMID: 32341345) ; Science. 2015 Jan 30;347(6221):543-8. (PMID: 25592419) ; Nat Commun. 2018 Mar 14;9(1):1066. (PMID: 29540691) ; Cell. 2014 Nov 6;159(4):896-910. (PMID: 25417164) ; J Oral Maxillofac Pathol. 2012 Sep;16(3):400-5. (PMID: 23248474) ; PLoS One. 2023 Mar 29;18(3):e0283159. (PMID: 36989279) ; Methods Mol Biol. 2011;785:109-22. (PMID: 21901596) ; J Pathol Transl Med. 2016 Nov;50(6):411-418. (PMID: 27809448) ; Nat Methods. 2005 Dec;2(12):920-31. (PMID: 16299477) ; Nat Rev Methods Primers. 2021;1(1):. (PMID: 35128463) ; BMC Biol. 2023 May 24;21(1):113. (PMID: 37221592) ; Br J Cancer. 2018 Apr;118(7):995-999. (PMID: 29515257) ; J Neurochem. 2017 Aug;142 Suppl 2:130-143. (PMID: 28791703) Grant Information: NSTC 111-2636-B-007-007 National Science and Technology Council; NSTC 07-2314-B-007-003-MY3 National Science and Technology Council; NSTC 110-2321-B-010-006 National Science and Technology Council; MOST 110-2321-B-010-006 National Science and Technology Council; NSTC-111-2321-B-002-016 National Science and Technology Council Contributed Indexing: Keywords: FFPE; archived tissues; human; mouse; multiplexed staining; neuroscience; tissue clearing; whole brain analysis; whole mouse brain imaging Molecular Sequence: Dryad 10.5061/dryad.prr4xgxv7 Entry Date(s): Date Created: 20240522 Date Completed: 20240522 Latest Revision: 20240524 Update Code: 20240524 PubMed Central ID: PMC11111220

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.