Analysis of the cya locus of Escherichia coli.
In: Gene, Jg. 28 (1984-05-01), Heft 2, S. 133-46
academicJournal
Zugriff:
A 9500-bp DNA segment containing the adenylate cyclase gene (cya) of Escherichia coli has been isolated and analyzed. Four large proteins are encoded within this fragment - the adenylate cyclase protein (92 kDal), two proteins of unknown function (37 and 32 kDal), and a part of the uvrD-coded protein. Various truncated adenylate cyclase proteins, made from cya genes having as much as 60% of their carboxy-terminal end deleted, are sufficient to complement cya- hosts. When these truncated cya genes are present on a multicopy plasmid in a cya- host, the synthesis of beta-galactosidase is still regulated by glucose. The "maxicell" technique was used to visualize the four proteins encoded by this region and some of the truncated adenylate cyclase proteins.
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Analysis of the cya locus of Escherichia coli.
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Autor/in / Beteiligte Person: | Koop, AH ; Hartley, M ; Bourgeois, S |
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Zeitschrift: | Gene, Jg. 28 (1984-05-01), Heft 2, S. 133-46 |
Veröffentlichung: | Amsterdam, Elsevier/North-Holland, 1976-, 1984 |
Medientyp: | academicJournal |
ISSN: | 0378-1119 (print) |
DOI: | 10.1016/0378-1119(84)90250-6 |
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