Malate dehydrogenase and glucose-6-phosphate dehydrogenase, key markers for studying the genetic diversity of Actinobacillus actinomycetemcomitans.
In: FEMS microbiology letters, Jg. 122 (1994-09-15), Heft 1-2, S. 69-73
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Zugriff:
Cell-free extracts of strains belonging to the 5 serotypes of A. actinomycetemcomitans were screened for several enzymes. Enzymes representative of the pentose phosphate pathway/hexose monophosphate shunt and the TCA cycle were present. Of these glucose-6-phosphate dehydrogenase (G6PDH) and malate dehydrogenase (MDH) were the most readily detected and stable. MDH and G6PDH retained more than 50% of their activities at alkaline pHs (10-11) for up to 6 h and 3 h at 25 degrees C, respectively, while at pH 6.5, 50% of their activities were lost within 2-3 h. The Km for malate oxidation catalysed by MDH was 5.8 x 10(-4) M while that for glucose-6-phosphate oxidation was 2.0 x 10(-4) M. The pH optima for MDH and G6PDH oxidation activities were 10 and 9.5, respectively. Among the 5 designated serotypes of A. actinomycetemcomitans three groups were delineated by multilocus enzyme electrophoresis using MDH and G6PDH.
| Titel: |
Malate dehydrogenase and glucose-6-phosphate dehydrogenase, key markers for studying the genetic diversity of Actinobacillus actinomycetemcomitans.
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| Autor/in / Beteiligte Person: | Shah, HN ; Andrews, DM |
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| Zeitschrift: | FEMS microbiology letters, Jg. 122 (1994-09-15), Heft 1-2, S. 69-73 |
| Veröffentlichung: | 2015- : Oxford Oxford University Press ; <i>Original Publication</i>: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies., 1994 |
| Medientyp: | academicJournal |
| ISSN: | 0378-1097 (print) |
| DOI: | 10.1111/j.1574-6968.1994.tb07145.x |
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