Characterization of DBT cell clones derived from cells persistently infected with the JHM strain of mouse hepatitis virus.

Maeda, A ; Hayashi, M ; et al.

In: The Journal of veterinary medical science, Jg. 57 (1995-10-01), Heft 5, S. 813-7

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Twelve clones derived from the cells persistently infected with the JHM strain (JHMV) of mouse hepatitis virus (MHV) were established from mouse astrocytoma-derived DBT cells and characterized. All the cell clones were resistant to superinfection with MHV. Only one of the persistently infected cell clone synthesized viral RNA and proteins and produced virus particles. Viral RNA was detectable in some other cell clones without production of viral protein nor the virus. No cell clones exhibited contact fusion activity. The results suggested that such variety of cell clones might have resulted from persistent infection with JHMV.

Titel:	Characterization of DBT cell clones derived from cells persistently infected with the JHM strain of mouse hepatitis virus.
Autor/in / Beteiligte Person:	Maeda, A ; Hayashi, M ; Ishida, K ; Mizutani, T ; Watanabe, T ; Namioka, S
Link:	Full Text Finder (Volltext)
Zeitschrift:	The Journal of veterinary medical science, Jg. 57 (1995-10-01), Heft 5, S. 813-7
Veröffentlichung:	Tokyo : Japanese Society Of Veterinary Science, 1995
Medientyp:	academicJournal
ISSN:	0916-7250 (print)
DOI:	10.1292/jvms.57.813
Schlagwort:	Animals Astrocytoma pathology Astrocytoma virology Base Sequence Brain Chemistry Brain Neoplasms pathology Brain Neoplasms virology Clone Cells Coronavirus Infections pathology Coronavirus Infections virology DNA Primers analysis DNA Primers chemistry DNA Primers genetics DNA, Viral analysis DNA, Viral chemistry DNA, Viral genetics Mice Molecular Sequence Data Murine hepatitis virus genetics Mutation Polymerase Chain Reaction veterinary RNA, Viral analysis RNA, Viral chemistry RNA, Viral genetics Rodent Diseases genetics Rodent Diseases virology Tumor Cells, Cultured Viral Plaque Assay veterinary Astrocytoma veterinary Brain Neoplasms veterinary Coronavirus Infections veterinary Murine hepatitis virus physiology Rodent Diseases pathology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Vet Med Sci] 1995 Oct; Vol. 57 (5), pp. 813-7. MeSH Terms: Astrocytoma / veterinary ; Brain Neoplasms / veterinary ; Coronavirus Infections / veterinary ; Murine hepatitis virus / physiology ; Rodent Diseases / *pathology ; Animals ; Astrocytoma / pathology ; Astrocytoma / virology ; Base Sequence ; Brain Chemistry ; Brain Neoplasms / pathology ; Brain Neoplasms / virology ; Clone Cells ; Coronavirus Infections / pathology ; Coronavirus Infections / virology ; DNA Primers / analysis ; DNA Primers / chemistry ; DNA Primers / genetics ; DNA, Viral / analysis ; DNA, Viral / chemistry ; DNA, Viral / genetics ; Mice ; Molecular Sequence Data ; Murine hepatitis virus / genetics ; Mutation ; Polymerase Chain Reaction / veterinary ; RNA, Viral / analysis ; RNA, Viral / chemistry ; RNA, Viral / genetics ; Rodent Diseases / genetics ; Rodent Diseases / virology ; Tumor Cells, Cultured ; Viral Plaque Assay / veterinary Substance Nomenclature: 0 (DNA Primers) ; 0 (DNA, Viral) ; 0 (RNA, Viral) Entry Date(s): Date Created: 19951001 Date Completed: 19960409 Latest Revision: 20190516 Update Code: 20240513

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