Enhanced tumor-forming capacity for immortalized melanocytes expressing melanoma growth stimulatory activity/growth-regulated cytokine beta and gamma proteins.
In: International journal of cancer, Jg. 73 (1997-09-26), Heft 1, S. 94-103
Online
academicJournal
Zugriff:
Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. We have previously demonstrated that MGSA/GRO alpha can be isolated from culture medium conditioned by malignant melanoma cells and that continuous secretion of MGSA/GRO alpha contributes to the transformation of immortalized murine melanocytes. The present study was designed to determine whether MGSA/GRO beta or -gamma have similar effects on melanocyte tumorigenicity. Stable Melan-a clones expressing either human MGSA/GRO beta or -gamma exhibited enhanced ability to form large colonies in soft agar and tumors in nude mice. The clones expressing the MGSA/GRO beta or -gamma transgene formed tumors within 2 months after injection; the tumors were highly pigmented and expressed immunoreactive MGSA/GRO beta or -gamma protein. Furthermore, when conditioned medium from Melan-a clones expressing MGSA/GRO alpha, -beta or -gamma transgenes were examined for the ability to induce angiogenesis in the rat cornea, strong angiogenic responses were observed. This angiogenic response was blocked by antibodies to the respective MGSA/GRO protein, but not by normal rabbit serum. By contrast, angiogenic responses were observed in only 2 of 12 corneal implants (17%) containing medium conditioned by Melan-a clones expressing the neomycin resistance marker alone.
Titel: |
Enhanced tumor-forming capacity for immortalized melanocytes expressing melanoma growth stimulatory activity/growth-regulated cytokine beta and gamma proteins.
|
---|---|
Autor/in / Beteiligte Person: | Owen, JD ; Strieter, R ; Burdick, M ; Haghnegahdar, H ; Nanney, L ; Shattuck-Brandt, R ; Richmond, A |
Link: | |
Zeitschrift: | International journal of cancer, Jg. 73 (1997-09-26), Heft 1, S. 94-103 |
Veröffentlichung: | 1995- : New York, NY : Wiley-Liss ; <i>Original Publication</i>: 1966-1984 : Genève : International Union Against Cancer, 1997 |
Medientyp: | academicJournal |
ISSN: | 0020-7136 (print) |
DOI: | 10.1002/(sici)1097-0215(19970926)73:1<94::aid-ijc15>3.0.co;2-5 |
Schlagwort: |
|
Sonstiges: |
|