The Effects of Exercise on the Lipid Profile of Extracellular Vesicles

We sought to ascertain the effect of different types of exercise (aerobic training and resistance training) on the lipid profile of extracellular vesicles. Thus, we had participants perform bouts of AT and RT and rest (control) and collected blood samples from them immediately after (timepoint 0) and 30 minutes after (timepoint 30) exercise. Small extracellular vesicles (sEVs) were isolated from plasma of participants using cushioned-density gradient ultracentrifugation (C-DGUC). Small EV size, morphology, and protein markers were determined using nanoparticle tracking analysis, transmission electron microscopy, and western blot, respectively. Additionally, we performed untargeted lipidomics on the sEV isolate. We found that RT significantly increases the size of sEVs immediately after exercise and that both types of exercise causes slight changes in the lipid profile of circulating sEVS. We specifically observed changes in Anandamide (AEA), Ceramide (Cer), Cholesterol Ester (ChE), Carboxymethyl ethanolamine (CmE), Trihexosylceramide (Hex3Cer), Lyso- phosphatidylglycerol (LPG) Lyso-phosphatidylinositol (LPI), Lyso-sphingomyelin (LSM), and Wax monoesters (WE). Advisor: Ivan Vechetti