Detection and Modulation of Biological Cues using DNA Origami Nanodevices
2023
Hochschulschrift
Zugriff:
DNA origami technology allows for the design and fabrication of biocompatible and 3-D functional nanodevices via molecular self-assembly for various biological applications including cell function regulation, disease therapy and biomolecular detection. Biocompatibility and programmability of DNA molecules lead into the development of complex and functional DNA nanodevices that can work in biological media and living systems. Stability and functionality of DNA nanodevices compared to DNA molecules itself, make it an effective approach for cell modulation and payload delivery. The field of nanomedicine, has developed a path to detect and fight infectious disease using nanomaterials and DNA nanostructures are novel additions to the nanomaterial collection, providing great progress in diagnostics and therapy. Nucleic acids, proteins and whole pathogens are among the viral and bacterial targets which can be detected using DNA nanostructures.In this dissertation, we report four studies on biological applications of DNA origami in detection and modulation of biological cues. We establish the successful incorporation of DNA nanodevices on the cell membrane and report a robust method to monitor live cell interactions with biomolecules in their surrounding environment. Our results establish the integration of live cells with membranes engineered with DNA nanodevices into microfluidic chips as a highly capable biosensor approach to investigate subcellular interactions in physiologically relevant 3-D environments under controlled biomolecular transport. Moreover, we propose a novel complex DNA platform for modulating the gap in intercellular junctions and finally establish three DNA nanodevices for the detection of viral and bacterial nucleic acids in human samples. Collectively, the findings of this dissertation establish a foundation on functionality of nanodevices for biological applications.
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Detection and Modulation of Biological Cues using DNA Origami Nanodevices
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Autor/in / Beteiligte Person: | Shahhosseini, Melika |
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Veröffentlichung: | 2023 |
Medientyp: | Hochschulschrift |
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