Investigating the role of 5-HT and MCH in the regulation of human immune response
2020
Hochschulschrift
Zugriff:
The involvement of neurotransmitters and neuropeptides as mediators in the dysregulation of the immune response leading to conditions such as chronic inflammation, autoimmune diseases and haematological cancers has been reported. Indeed, different types of innate and adaptive immune cells have been shown to express neurotransmitter/neuropeptide receptors on their surface membrane and some are even able to produce the neural factors under different conditions. This project was designed to investigate the role of the monoamine serotonin (5- HT) and also that of the orexigenic neuropeptide melanin-concentrating hormone (MCH) in human immune cell functions, specifically T lymphocytes, one of the key players in adaptive immune and inflammatory responses. To investigate the effects of agonist and antagonists targeting 5-HT receptors on the CD4+ and CD8+ T cell response to stimulus, human peripheral blood mononuclear cells were stimulated to induce proliferation in a serum-free environment. It was observed that inhibition of 5-HT synthesis enzyme tryptophan hydroxylase 1 (TPH1) with para-chlorophenylalanin (pCPA; 0.1-1mM) suppressed proliferation of both CD4 and CD8 T lymphocytes with significant inhibition observed at 1mM (p
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Investigating the role of 5-HT and MCH in the regulation of human immune response
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Autor/in / Beteiligte Person: | Yusoff, Syaratul Dalina |
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Veröffentlichung: | 2020 |
Medientyp: | Hochschulschrift |
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