The effect of α-aminoadipate δ-semialdehyde synthase knockdown on the lysine requirement and urate oxidase knockdown on oxidative stress in a murine hepatic cell line.
2007
Hochschulschrift
Zugriff:
The technique of RNA interference was applied in two different systems. In system I, RNA interference was used to knockdown expression of α-aminoadipate δ-semialdehyde synthase (AASS). AASS is the bifunctional enzyme containing the lysine α-ketoglutarate reductase (LKR) and saccharopine dehydrogenase activities responsible for the first two steps in the irreversible catabolism of lysine. A rare disease in humans, familial hyperlysinemia, can be caused by very low LKR activity and, as expected, reduces the lysine “requirement†of the individual. This concept was recreated in a murine hepatic cell line (ATCC, FL83B) utilizing RNA interference to achieve AASS mRNA knockdown. Cells were antibiotic selected for stable transfection of two plasmids that express different short hairpin RNA sequences for AASS knockdown. Compared to the wild type cell line, AASS mRNA abundance was reduced 79.0 ± 6.4% (p
Titel: |
The effect of α-aminoadipate δ-semialdehyde synthase knockdown on the lysine requirement and urate oxidase knockdown on oxidative stress in a murine hepatic cell line.
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Autor/in / Beteiligte Person: | Cleveland, Beth Marie |
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Veröffentlichung: | 2007 |
Medientyp: | Hochschulschrift |
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