Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells.
In: Chinese Journal of Integrative Medicine, Jg. 24 (2018-03-01), Heft 3, S. 213-217
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Objective: To investigate the effects of Compound Zhebei Granule (复方浙贝颗粒, CZG) combined with doxorubicin hydrochloride (adriamycin, ADM) on specific surface antigens in mice with KG-1a transplanted cells.Methods: A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flfl anks of BALB/c-nude mice. Twenty-four tumor bearing mice were divided into 4 groups according to a random number table, including normal saline control group, ADM group, high-dose CZG group, and mid-dose CZG group, with 6 mice in each group. Drug administration occurred on the 14th day after cell inoculation, and normal saline control group mice were gavaged with normal saline at 0.2 mL/10 g every other day. ADM group mice were intraperitoneally injected with ADM 1 mg/kg [conversion of adults, 40 mg/(m 2 •d)] every other day. High- and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM (1 mg/kg) every other day. The administration period lasted for 10 days. The tumor xenografts were made into mononuclear cell suspensions after dissection, and the expressions of specific surface antigens, including CD34 + CD38 - , CD34 + CD38 - CD123 + , CD34 + CD38 - CD96 + and CD34 + CD38 - CD33 + , in KG-1a cell line tumor xenografts were detected by flfl ow cytometry.Results: Compared with the control and ADM groups, expression of CD34 + CD38 - in the two CZG groups was significantly lower ( P <0.05). Compared with the control group, expression of CD34 + CD38 - CD123 + in the two CZG groups decreased ( P <0.05). The high-dose CZG group showed more obvious outcomes compared with the ADM group ( P <0.05). Compared with the control and ADM groups, the expression of CD34 + CD38 - CD96 + and CD34 + CD38 - CD33 + in the two CZG groups decreased ( P <0.05).Conclusions: CZG combined with doxorubicin could reduce the expression of CD34 + CD38 - , CD34 + CD38 - CD123 + , CD34 + CD38 - CD96 + and CD34 + CD38 - CD33 + in KG-1a cell line tumor xenografts, which shows that CZG could target leukemia stem cells and play a role in chemosensitization. [ABSTRACT FROM AUTHOR]
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Titel: |
Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells.
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Autor/in / Beteiligte Person: | Zhang, Yu ; Hou, li ; Chen, Xin-yi |
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Zeitschrift: | Chinese Journal of Integrative Medicine, Jg. 24 (2018-03-01), Heft 3, S. 213-217 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 1672-0415 (print) |
DOI: | 10.1007/s11655-017-2963-1 |
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