Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice.
In: Science Translational Medicine, Jg. 11 (2019-03-20), Heft 484, S. N.PAG
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Zugriff:
Modulation of human amphiphysin 2 expression by genetic cross and viral delivery rescues all hallmarks of X-linked centronuclear myopathy in mice. A muscle-building interaction: Centronuclear myopathies (CNMs) are rare genetic disorders characterized by severe muscle weakness. Mutations in myotubularin 1 (MTM1) and in amphiphysin 2 (BIN1) are responsible for two different forms of the disease. BIN1 and MTM1 have been shown to interact in skeletal muscles. Now, Lionello et al. investigated the role of this interaction in a model of Mtm1-mediated CNM. Postnatal BIN1 overexpression improved survival and muscle strength in Mtm1 knockout mice. The treatment also restored myofiber integrity and rescued extracellular matrix and focal adhesion defects in myofibers. The results suggest that BIN1-MTM1 interaction plays a role in CNM and could be targeted for treating CNM due to MTM1 mutations. Centronuclear myopathies (CNMs) are severe diseases characterized by muscle weakness and myofiber atrophy. Currently, there are no approved treatments for these disorders. Mutations in the phosphoinositide 3-phosphatase myotubularin (MTM1) are responsible for X-linked CNM (XLCNM), also called myotubular myopathy, whereas mutations in the membrane remodeling Bin/amphiphysin/Rvs protein amphiphysin 2 [bridging integrator 1 (BIN1)] are responsible for an autosomal form of the disease. Here, we investigated the functional relationship between MTM1 and BIN1 in healthy skeletal muscle and in the physiopathology of CNM. Genetic overexpression of human BIN1 efficiently rescued the muscle weakness and life span in a mouse model of XLCNM. Exogenous human BIN1 expression with adeno-associated virus after birth also prevented the progression of the disease, suggesting that human BIN1 overexpression can compensate for the lack of MTM1 expression in this mouse model. Our results showed that MTM1 controls cell adhesion and integrin localization in mammalian muscle. Alterations in this pathway in Mtm1 −/y mice were associated with defects in myofiber shape and size. BIN1 expression rescued integrin and laminin alterations and restored myofiber integrity, supporting the idea that MTM1 and BIN1 are functionally linked and necessary for focal adhesions in skeletal muscle. The results suggest that BIN1 modulation might be an effective strategy for treating XLCNM. [ABSTRACT FROM AUTHOR]
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Titel: |
Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice.
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Autor/in / Beteiligte Person: | Lionello, Valentina M. ; Nicot, Anne-Sophie ; Sartori, Maxime ; Kretz, Christine ; Kessler, Pascal ; Buono, Suzie ; Djerroud, Sarah ; Messaddeq, Nadia ; Koebel, Pascale ; Prokic, Ivana ; Hérault, Yann ; Romero, Norma B. ; Laporte, Jocelyn ; Cowling, Belinda S. |
Zeitschrift: | Science Translational Medicine, Jg. 11 (2019-03-20), Heft 484, S. N.PAG |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1946-6234 (print) |
DOI: | 10.1126/scitranslmed.aav1866 |
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