Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in In Vivo and In Vitro Models of Hypoxia.
In: Frontiers in Pharmacology, 2019-09-26, S. 1-14
Online
academicJournal
Zugriff:
Zhenlong Xingnao Capsule (ZXC) is a Tibetan medicine used to treat ischemic stroke. In this study, we determined the in vitro and in vivo effects of ZXC on reactive oxygen species (ROS) in a mouse BV-2 microglial cell hypoxia-reoxygenation and rat middle cerebral artery occlusion infarction models. We aimed to clarify the role of ZXC in cerebral ischemia protection; reveal amino acid neurotransmitter changes in the frontal cortex after drug intervention; determine mRNA and protein expression changes in Bcl-2, Bax, caspase-3, P38, and nuclear factor (NF)-кB in the frontal cortex and changes in antioxidant indices in the brain; and elucidate the mechanisms underlying ZXC action. After hypoxia-reoxygenation, ROS levels were significantly increased in BV-2 cells, and their levels decreased after treatment with ZXC. ZXC had protective effects on ischemic/anoxic injury in vitro and in vivo by downregulating the expressions of caspase-3 and NF-кB mRNA during ischemia and reperfusion and that of p38 and caspase-3 during acute ischemia and reperfusion as well as the steady-state levels of excitatory amino acids/inhibitory amino acids and by improving the total antioxidant capacity and total superoxide dismutase activities during ischemia. These findings provide new molecular evidence for the mechanisms underlying ZXC action. [ABSTRACT FROM AUTHOR]
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Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in In Vivo and In Vitro Models of Hypoxia.
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Autor/in / Beteiligte Person: | Wei, Xia ; Zhu, Qingfen ; Liu, Na ; Xu, Lihua ; Wei, Sheng ; Fan, Zhiyun ; Sun, Changhua ; Zhao, Yan ; Qiao, Mingqi ; Wu, Jibiao ; Hu, Defu ; Wang, Yang ; Sun, Peng |
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Zeitschrift: | Frontiers in Pharmacology, 2019-09-26, S. 1-14 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1663-9812 (print) |
DOI: | 10.3389/fphar.2019.01096 |
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