mCIM法与PAE-MHT法检测铜绿假单胞歯 产金β-内酰胺酶的性能评价. (Chinese)

Objective To evaluate the screening capacity of modified carbapenem inactivation method(mCIM) and Pseudomonas aeruginosa-Moeified Hodge(PAE-MHT) test for detection of metallo-p-lactamase-producing(MBLs) in PAE. Methods 176 carbapenem-resistant PAE clinical isolates were dectected to MBLs by mCIM and PAE-MHT test, respectively. Meanwhile, IMP genes and VIM genes were detected by PCR. TTie examination results were analyzed statistically. Results Comparing mCIM of imipenem-substrate methods to mCIM of meropenem-substrate method, the dectction rates of MBLs were not statistically significant= 0. 01, P > 0. 05). For mCIM of imipenem-substrate, mCIM of meropenem-substrate and PAEMHT，the accuracy rate was 94.9%，96. 6% and 94. 3%，respectively. TTie sensitivity was 90.5%，100% and 85.7%，respectively. The specificity was 95.5%, 96.1% and 95.5%, respectively. Pairwise comparison of accuracy, sensitivity and specificity among the three groups showed no statistically significant difference = 0• 001〜2. 99，all P > 0• 05) • Three methods had a good consistency compared with PCR . Conclusion PAE-MHT test is a method of economical, practical and simple operation. TTie mCIM of meropenem-substrate is high sensitivity and good specificity for detecting MBLs-producing PAE. [ABSTRACT FROM AUTHOR]

目的评估改良的碳青霉烯类灭活法（mCIM)和铜绿假单胞菌改良Hodge试验（PAE-MHT)法对铜绿假单胞 菌（PAE)产金属p-内酰胺酶（MBLs)的表型筛选能力.:方法收集176株耐碳青霉烯类PAE,PCR检测IMP和VIM 基因，采用mCIM法和PAE-MHT法检测产MBLs菌株，其中mCIM法分别选择亚胺培南（IMP)和美罗培南（MEM)作 为药物底物进行筛选能力的研究，对几种方法与PCR检测结果的一致性进行统计分析结果以IMP为底物mCIM 法和以1为底物111(11«法检测\!818的阳性率差异无统计学意义(X 2 =0.01，户>0.05)。以11«?为底物111(:11«法、 以MEM为底物mCIM法和PAE-MHT法的准确度分别为94.9%, 96.6%和94.3% ;敏感度分别为90.5%, 100%和 85.7% ;特异度分别为95.5%, 96.1%和95.5%。三者在准确度、敏感度和特异度之间两两相比，差异均无统计学意 义(尤2=0.01~2.99,均P>0.05)。三种方法与PCR法均具有良好的一致性。结论 PAE-MHT法经济、操作简便， 结果易于判读。以MEM为底物的mCIM法具有高敏感度与高特异度，更适合于检测PAE的MBLs。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)