Suspension culture promotes serosal mesothelial development in human intestinal organoids.
In: Cell Reports, Jg. 38 (2022-02-15), Heft 7, S. N.PAG
academicJournal
Zugriff:
Pluripotent-stem-cell-derived human intestinal organoids (HIOs) model some aspects of intestinal development and disease, but current culture methods do not fully recapitulate the diverse cell types and complex organization of the human intestine and are reliant on 3D extracellular matrix or hydrogel systems, which limit experimental control and translational potential for regenerative medicine. We describe suspension culture as a simple, low-maintenance method for culturing HIOs and for promoting in vitro differentiation of an organized serosal mesothelial layer that is similar to primary human intestinal serosal mesothelium based on single-cell RNA sequencing and histological analysis. Functionally, HIO serosal mesothelium has the capacity to differentiate into smooth-muscle-like cells and exhibits fibrinolytic activity. An inhibitor screen identifies Hedgehog and WNT signaling as regulators of human serosal mesothelial differentiation. Collectively, suspension HIOs represent a three-dimensional model to study the human serosal mesothelium. [Display omitted] • Suspension culture supports growth of hPSC-derived human intestinal organoids (HIOs) • Suspension HIOs form an outer serosal mesothelial layer • HIO serosa is molecularly and functionally similar to human intestinal serosa • HH and WNT signaling play a role in differentiation and patterning of the serosa Capeling et al. describe suspension culture for human intestinal organoids as an alternative to Matrigel. Suspension organoids develop a serosal mesothelial layer that resembles the serosa of the human fetal intestine. Using this system, HH and WNT signaling are implicated in differentiation and patterning of the human intestinal serosal mesothelium. [ABSTRACT FROM AUTHOR]
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Titel: |
Suspension culture promotes serosal mesothelial development in human intestinal organoids.
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Autor/in / Beteiligte Person: | Capeling, Meghan M. ; Huang, Sha ; Childs, Charlie J. ; Wu, Joshua H. ; Tsai, Yu-Hwai ; Wu, Angeline ; Garg, Neil ; Holloway, Emily M. ; Sundaram, Nambirajan ; Bouffi, Carine ; Helmrath, Michael ; Spence, Jason R. |
Zeitschrift: | Cell Reports, Jg. 38 (2022-02-15), Heft 7, S. N.PAG |
Veröffentlichung: | 2022 |
Medientyp: | academicJournal |
ISSN: | 2639-1856 (print) |
DOI: | 10.1016/j.celrep.2022.110379 |
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