Volltext verfügbar nach Anmeldung bzw. im Campus-Netz.
Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study.
In: Cancers, Jg. 14 (2022-09-01), Heft 17, S. 4343-4355
Online
academicJournal
Zugriff:
Simple Summary: We conducted a multicenter validation study using a methylation assay, named MPap, to detect EC. MPap is used to identify the DNA methylation status of two genes, BHLHE22 and CDO1, from cervical scrapings, and the results are combined with age and body mass index. In two stages of validation, the sensitivity, specificity, and positive and negative predictive values were 92.5~92.9%, 71.5~73.8%, 39.8~40.2%, and 98.0~98.1%, respectively. The MPap test is a feasible alternative tool that provides physicians with a reference for assessing susceptibility to endometrial cancer. Background: We describe a DNA methylation assay, named MPap test, using cervical scraping as an alternative technique for endometrial cancer detection. Methods: A multicenter hospital-based, two-stage validation study was conducted to validate the cancer detection performance of the MPap test. The MPap value was determined from the DNA methylation status of two genes (BHLHE22, CDO1) and combined with two other clinical variables (age, BMI). The cutoff threshold of the MPap value was established in stage 1 and validated in stage 2. A total of 592 women with abnormal uterine bleeding were enrolled from five medical centers throughout Taiwan. Results: In stage 1, the sensitivity, specificity, and positive and negative predictive values of the MPap test for detecting endometrial cancer were 92.9%, 71.5%, 39.8%, and 98.0%, respectively. These values were validated in stage 2, being 92.5%, 73.8%, 40.2%, and 98.1%. Moreover, MPap outperformed transvaginal ultrasound in sensitivity and negative predictive values for detecting endometrial cancer. When we applied the algorithm for triage of endometrial cancer detection by MPap in the Taiwan National Health Insurance dataset, we found that it may reduce invasive procedures by 69~73%. Conclusions: MPap may provide a feasible alternative for endometrial cancer detection and can be considered as a triage test to reduce unnecessary invasive procedures. [ABSTRACT FROM AUTHOR]
Copyright of Cancers is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study.
|
---|---|
Autor/in / Beteiligte Person: | Wen, Kuo-Chang ; Huang, Rui-Lan ; Chen, Lin-Yu ; Wu, Tzu-I ; Lu, Chien-Hsing ; Chu, Tang-Yuan ; Ou, Yu-Che ; Wu, Chen-Hsuan ; Hsu, Shih-Tien ; Ding, Dah-Ching ; Chu, Ling-Hui ; Chen, Chien-Wen ; Chang, Heng-Cheng ; Liu, Yu-Shu ; Wang, Hui-Chen ; Weng, Yu-Chun ; Su, Po-Hsuan ; Lin, Hao ; Lai, Hung-Cheng |
Link: | |
Zeitschrift: | Cancers, Jg. 14 (2022-09-01), Heft 17, S. 4343-4355 |
Veröffentlichung: | 2022 |
Medientyp: | academicJournal |
ISSN: | 2072-6694 (print) |
DOI: | 10.3390/cancers14174343 |
Schlagwort: |
|
Sonstiges: |
|