Differential Amino Acid Uptake and Depletion in Mono-Cultures and Co-Cultures of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in a Novel Semi-Synthetic Medium.
In: Microorganisms, Jg. 10 (2022-09-01), Heft 9, S. 1771-1786
Online
academicJournal
Zugriff:
The mechanistic understanding of the physiology and interactions of microorganisms in starter cultures is critical for the targeted improvement of fermented milk products, such as yogurt, which is produced by Streptococcus thermophilus in co-culture with Lactobacillus delbrueckii subsp. bulgaricus. However, the use of complex growth media or milk is a major challenge for quantifying metabolite production, consumption, and exchange in co-cultures. This study developed a synthetic medium that enables the establishment of defined culturing conditions and the application of flow cytometry for measuring species-specific biomass values. Time courses of amino acid concentrations in mono-cultures and co-cultures of L. bulgaricus ATCC BAA-365 with the proteinase-deficient S. thermophilus LMG 18311 and with a proteinase-positive S. thermophilus strain were determined. The analysis revealed that amino acid release rates in co-culture were not equivalent to the sum of amino acid release rates in mono-cultures. Data-driven and pH-dependent amino acid release models were developed and applied for comparison. Histidine displayed higher concentrations in co-cultures, whereas isoleucine and arginine were depleted. Amino acid measurements in co-cultures also confirmed that some amino acids, such as lysine, are produced and then consumed, thus being suitable candidates to investigate the inter-species interactions in the co-culture and contribute to the required knowledge for targeted shaping of yogurt qualities. [ABSTRACT FROM AUTHOR]
Copyright of Microorganisms is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Differential Amino Acid Uptake and Depletion in Mono-Cultures and Co-Cultures of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in a Novel Semi-Synthetic Medium.
|
---|---|
Autor/in / Beteiligte Person: | Ulmer, Andreas ; Erdemann, Florian ; Mueller, Susanne ; Loesch, Maren ; Wildt, Sandy ; Jensen, Maiken Lund ; Gaspar, Paula ; Zeidan, Ahmad A. ; Takors, Ralf |
Link: | |
Zeitschrift: | Microorganisms, Jg. 10 (2022-09-01), Heft 9, S. 1771-1786 |
Veröffentlichung: | 2022 |
Medientyp: | academicJournal |
ISSN: | 2076-2607 (print) |
DOI: | 10.3390/microorganisms10091771 |
Sonstiges: |
|