福建省猪源多黏菌素耐药基因 mcr-1 阳性细菌的 耐药特性及分布特征. (Chinese)

[Objective] This study aims at understanding the resistance characteristics and distribution of mcr-1 gene positive bacteria in Fujian.[Method]A total of 313 fecal samples from 21 pig farms in 7 cities of Fujian were collected, and mcr-1 gene positive bacteria was screened, isolated and identified by PCR. The drug sensitive was tested by K-B agar diffusion test, and the resistance phenotype was analyzed by PCR method. The sequence type (ST) of mcr~l gene positive E. coli was determined by multilocus sequence typing (MLST) method, and cluster analysis using pulsed field gel electrophoresis (PFGE) was performed to determine the distribution and phylogenetic relationships of mcr-l gene positive E. coli.[Result]A total of 43 strains of mc/-1 gene positive bacteria were isolated from 313 pig manure, including 39 strains of E. coli. The drug sensitive test showed that the resistance rates of these isolates to sulfamethoxazole, florfeni-col, streptomycin, doxycycline, enrofloxacin, neomycin, amoxicillin, cefotaxime Jin co spectacular, imipenem, nitrofurantoin, linezolid and tegacyclin were 90. 1 %, 83. 7 %, 74. 4%, 67. 4%, 67. 4%, 58. 1 %, 53. 5 %, 39. 5 %, 34. 9 %, 11. 6% and 4. 6%,0 and 0, respectively. The detection rates of sul\, sulZ and sul3 genes were 81. 4%, 90. 7% and 74. 4%, respeclively. Among quinolone resistance genes, only qnrs and aac(.6f)-Ib~ cr were detected with detection rates of 51. 2% and 30. 2%. The detection rates of Cat2 and cm IA genes in chloramphenicol resistance genes were 86. 0% and 74. 4%. The detection rate of florfenicol resistance gene was 83. 7 %. The detection rates of NDM-1 and cfr gene were 23. 2% and 7. 0%. The tegacyclin resistance gene (仏£(X)) was not detected. Among the 39 strains, 35 of gene positive E. coli with high diversity were divided into 19 ST types. The ST10 type was dominant with a total of 9 strains. PFGE map showed that these 39 mcr~l gene positive E. coli obtained 30 PFGE patterns in 6 clone groups with polymorphic characteristics.[Conclusion] The mc/-1 gene positive bacteria from swine in Fujian were mainly E. coli with multiple drug resistance and a few were other strains of Enterobacteriaceae. The mcr~l gene positive E. coli showed characteristics of polymorphism and high diversity in regional distribution. [ABSTRACT FROM AUTHOR]

[目的]了解福建省猪源尬厅1基因阳性细菌的流行性、耐药特性及分布特征。[方法]从福建省7个 地市的21个猪场采集313份粪便样本, 应用PCR方法筛选、分离和鉴定加纣1基因阳性细菌;采用K B琼脂扩散试 验进行药敏检测, 分析观基因阳性细菌的耐药性,并使用PCR方法分析其耐药表型;进一步采用多位点序列分型 (MLST)方法鉴定mcr 1基因阳性大肠杆菌的类型,使用脉冲场凝胶电泳(PFGE)分析方法对mcr 1阳性大肠杆菌进 行聚类分析, 探明其分布特征和亲缘关系。[结果]从313份猪粪便中共分离获得43株mcr 1基因阳性细菌, 其中大 肠杆菌39株。耐药性结果显示, 分离菌株对磺胺甲基异恶輕、氟苯尼考、链霉素、强力霉素、恩诺沙星、新霉素、阿莫西 林、头抱嗟弱、林可壮观、亚胺培南、咲喃妥因、利奈睦胺和替加环素的耐药率分别为90. 1%,83. 7%,74. 4%,67. 4%, 67. 4%, 5& 1 %, 53. 5%, 39. 5%, 34. 9%, 11. 6%, 4. 6%, 0 和 0。磺胺类耐药基因中的 sul\.sul2 和 sul3 基因检出率较 高, 分别达到81. 4%,90. 7%和74. 4%;唾诺酮类耐药基因中仅有炉拓和aac(6')必ct•被检出, 其检出率分别为 51.2%和30.2%;氯霉素类耐药基因中Cm2和cmfA基因的检出率较高, 分别达到86.0%和74.4%;氟苯尼考耐药 基因(夭。氏)的检出率为83.7%;金属p内酰胺酶耐药基因(N DM1)的检出率为23.2%;多药耐药基因(cR)的检出 率为7.0%;而替加环素耐药基因(tet(X))未被检出。39株观"1基因阳性大肠杆菌除了 4株不能分型外, 其他35 株共分为19个ST型, 具有高度多样性, 其中ST10为优势型, 共有9株菌株。PFGE图谱显示, 39株观纣1基因阳性 大肠杆菌共获得30种PFGE谱型, 具有多态性特征, 分为6组克隆群。[结论]福建猪源加纣1基因阳性细菌主要为 大肠杆菌,少部分为肠杆菌科其他菌株, 且分离菌株具有明显的多重耐药性;加基因阳性大肠杆菌在地域分布上 具有多态性和高度多样性特点。 [ABSTRACT FROM AUTHOR]

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