Volltext verfügbar nach Anmeldung bzw. im Campus-Netz.
Three R2R3-MYB transcription factors from banana (Musa acuminata) activate structural anthocyanin biosynthesis genes as part of an MBW complex.
In: BMC Research Notes, Jg. 16 (2023-06-13), Heft 1, S. 1-9
Online
academicJournal
Zugriff:
Objective: Bananas are one of the most popular fruits in the world, providing food security and employment opportunities in several developing countries. Increasing the anthocyanin content of banana fruit could improve the health-promoting properties. Anthocyanin biosynthesis is largely regulated at the transcriptional level. However, relatively little is known about the transcriptional activation of anthocyanin biosynthesis in banana. Results: We analysed the regulatory activity of three Musa acuminata MYBs that were predicted by bioinformatic analysis to transcriptionally regulate anthocyanin biosynthesis in banana. MaMYBA1, MaMYBA2 and MaMYBPA2 did not complement the anthocyanin-deficient phenotype of the Arabidopsis thaliana pap1/pap2 mutant. However, co-transfection experiments in A. thaliana protoplasts showed that MaMYBA1, MaMYBA2 and MaMYBPA2 function as components of a transcription factor complex with a bHLH and WD40 protein, the so called MBW complex, resulting in the activation of the A. thaliana ANTHOCYANIDIN SYNTHASE and DIHYDROFLAVONOL 4-REDUCTASE promoters. The activation potential of MaMYBA1, MaMYBA2 and MaMYBPA2 was increased when combined with the monocot Zea mays bHLH ZmR instead of the dicot AtEGL3. This work paves the path towards decoding the MBW complex-mediated transcriptional activation of anthocyanin biosynthesis in banana. It will also facilitate research towards increased anthocyanin content in banana and other monocot crops. [ABSTRACT FROM AUTHOR]
Copyright of BMC Research Notes is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Three R2R3-MYB transcription factors from banana (Musa acuminata) activate structural anthocyanin biosynthesis genes as part of an MBW complex.
|
---|---|
Autor/in / Beteiligte Person: | Busche, Mareike ; Pucker, Boas ; Weisshaar, Bernd ; Stracke, Ralf |
Link: | |
Zeitschrift: | BMC Research Notes, Jg. 16 (2023-06-13), Heft 1, S. 1-9 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 1756-0500 (print) |
DOI: | 10.1186/s13104-023-06375-2 |
Schlagwort: |
|
Sonstiges: |
|