PURIFICATION AND CHARACTERIZATION OF A MALATE DEHYDROGENASE FROMPHASEOLUS MUNGO.
In: Journal of Food Biochemistry, Jg. 29 (2005-04-01), Heft 2, S. 117-131
Online
academicJournal
Zugriff:
A malate dehydrogenase (MDH) was identified and isolated from the seeds of the mung bean (Phaseolus mungo). The procedure entailed extraction, ammonium sulfate precipitation, ion exchange chromatography on CM-Sephadex and high performance liquid chromatography on POROS HS-20. The purified protein exhibited a molecular mass of 38 kDa in SDS-polyacrylamide gel electrophoresis under both nonreduced and reduced conditions. The pI was 9.7 by isoelectric focusing. The specific activity of the MDH was estimated to be 199 U/mg. The enzyme expressed its optimum activity at pH 7.2, 35C, and showed stable activity below 40C. The Km for oxaloacetate was 112 µM. The partial N-terminal amino acid sequence data analysis of the first 20 amino acids of the mung bean MDH revealed 95 and 80% homology with two reported MDH from soya bean (Glycine max) and potato (Solanum tuberosum), respectively. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Food Biochemistry is the property of Hindawi Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
PURIFICATION AND CHARACTERIZATION OF A MALATE DEHYDROGENASE FROMPHASEOLUS MUNGO.
|
---|---|
Autor/in / Beteiligte Person: | Wang, Shaoyun ; Xu, Zhibin ; Ye, Xiuyun ; Rao, Pingfan |
Link: | |
Zeitschrift: | Journal of Food Biochemistry, Jg. 29 (2005-04-01), Heft 2, S. 117-131 |
Veröffentlichung: | 2005 |
Medientyp: | academicJournal |
ISSN: | 0145-8884 (print) |
DOI: | 10.1111/j.1745-4514.2005.00017.x |
Schlagwort: |
|
Sonstiges: |
|