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CD44-SNA1 integrated cytopathology for delineation of high grade dysplastic and neoplastic oral lesions.
In: PLoS ONE, Jg. 18 (2023-09-25), Heft 9, S. 1-24
Online
academicJournal
Zugriff:
The high prevalence of oral potentially-malignant disorders exhibits diverse severity and risk of malignant transformation, which mandates a Point-of-Care diagnostic tool. Low patient compliance for biopsies underscores the need for minimally-invasive diagnosis. Oral cytology, an apt method, is not clinically applicable due to a lack of definitive diagnostic criteria and subjective interpretation. The primary objective of this study was to identify and evaluate the efficacy of biomarkers for cytology-based delineation of high-risk oral lesions. A comprehensive systematic review and meta-analysis of biomarkers recognized a panel of markers (n: 10) delineating dysplastic oral lesions. In this observational cross sectional study, immunohistochemical validation (n: 131) identified a four-marker panel, CD44, Cyclin D1, SNA-1, and MAA, with the best sensitivity (>75%; AUC>0.75) in delineating benign, hyperplasia, and mild-dysplasia (Low Risk Lesions; LRL) from moderate-severe dysplasia (High Grade Dysplasia: HGD) along with cancer. Independent validation by cytology (n: 133) showed that expression of SNA-1 and CD44 significantly delineate HGD and cancer with high sensitivity (>83%). Multiplex validation in another cohort (n: 138), integrated with a machine learning model incorporating clinical parameters, further improved the sensitivity and specificity (>88%). Additionally, image automation with SNA-1 profiled data set also provided a high sensitivity (sensitivity: 86%). In the present study, cytology with a two-marker panel, detecting aberrant glycosylation and a glycoprotein, provided efficient risk stratification of oral lesions. Our study indicated that use of a two-biomarker panel (CD44/SNA-1) integrated with clinical parameters or SNA-1 with automated image analysis (Sensitivity >85%) or multiplexed two-marker panel analysis (Sensitivity: >90%) provided efficient risk stratification of oral lesions, indicating the significance of biomarker-integrated cytopathology in the development of a Point-of-care assay. [ABSTRACT FROM AUTHOR]
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CD44-SNA1 integrated cytopathology for delineation of high grade dysplastic and neoplastic oral lesions.
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Autor/in / Beteiligte Person: | Sunny, Sumsum P. ; D. R., Ravindra ; Hariharan, Aditi ; Mukhia, Nirza ; Gurudath, Shubha ; G., Keerthi ; Raghavan, Subhashini ; Kolur, Trupti ; Shetty, Vivek ; R., Vidya Bushan ; Surolia, Avadhesha ; T., Satyajit ; Chandrashekhar, Pavithra ; R., Nisheena ; Pandya, Hardik J. ; Pillai, Vijay ; N., Praveen Birur ; Kuriakose, Moni A. ; Suresh, Amritha |
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Zeitschrift: | PLoS ONE, Jg. 18 (2023-09-25), Heft 9, S. 1-24 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0291972 |
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