Melatonin dose-dependent effects on oocyte maturation capacity, in vitro fertilization and blastocyst development in mouse. (English)

Aims: Considering that melatonin is known as an effective ROS scavenger, it may be able to improve the invitro oocyte maturation and the fetal quality. This study was designed to determine the effect of melatonin on invitro oocyte maturation and fertilization and cleavage and blastocyst development rate in mice. Materials & Methods: This experimental study was carried out in the cellular-molecular center of Gilan Medical University in 2010. The cumulus-oocyte complex (COC) and germinal vesicle were obtained from mice. COCs were cultured for 18 hours in maturation medium (supplemented with different doses of melatonin (100, 10, 1 μM, 100, 10μM) and control group and their maturation rate was assessed. Then two pre-nucleus embryos were cultured in T6 medium supplemented with different doses of melatonin and embryos' developmental rate was recorded until blastocyst stage by inverted microscope. Data were analyzed using Chisquare test by SPSS 13 software. Results: 1, 10 and 100 μM concentrations of melatonin had positive effect on in-vitro oocyte maturation and fertilization in mice compared to the control group (p<0.01). In addition, the cleavage and blastocyst development rate increased in presence of 10and 100 nM of melatonin (p<0.01). Conclusion: Melatonin as an effective ROS scavenger has positive influence on in-vitro oocytes maturation and embryos' development promotion in mice. [ABSTRACT FROM AUTHOR]

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