No recombinations between Tcra-V and Tcra-C gene segments in 669 backcross mice.
In: Immunogenetics, Jg. 32 (1990-11-01), Heft 5, S. 297-303
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Zugriff:
Because T-cell receptor ( Tcr) genes may possibly function as non-major histocompatibility complex (MHC) immune response genes or predispose for autoimmune diseases, it is important to know how these genes are inherited. We found that Bgl I-digested DNA of BALB/c, C3H, DBA/2, and C57BL/6 exhibited restriction enzyme fragment length polymorphisms (RFLPs) for the Tcra-V1, Tcra-V2, Tcra-V4, Tcra-V6, Tcra-V7, Tcra-V8, Tcra-V11, Tcra-122, Tcra-V13, and Tcra-C gene segments. Inheritance of these RFLPs in 669 offspring from (BALB/c × C57BL/6) × BALB/c, (BALB/c × C57BL/6) × C57BL/6, (C57BL/6 × DBA2) × DBA/2, and (C57BL/6 × C3H) × C3H backcrosses was studied. Since we did not find any recombinations in the offspring, Tcra-V and Tcra-C gene segments are tightly linked and inherited as a haplotype. A peculiar finding was that 22 out of 103 (BALB/c × C57BL/6) × BALB/c offspring, heterozygous for Tcra-C, had deleted a C57BL/6 Tcra-V1 band as well as Tcra-V2 and Tcra-V4 bands. As will be discussed, this deletion is probably caused by heterogeneity in the C57BL/6 breeding stock of a commercial supplier. In seven BXD and BXH recombinant inbred strains with known recombinations between the Tcra-C and Es-10 loci, all Tcra-V RFLPs cosegregated with the Tcra-C RFLP. This finding agrees with the conclusion from our backcross studies; namely that Tcra-V and Tcra-C gene segments are tightly linked. [ABSTRACT FROM AUTHOR]
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Titel: |
No recombinations between Tcra-V and Tcra-C gene segments in 669 backcross mice.
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Autor/in / Beteiligte Person: | Gleditsch, Lars ; Snodgrass, Ralph ; Bogen, Bjarne |
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Zeitschrift: | Immunogenetics, Jg. 32 (1990-11-01), Heft 5, S. 297-303 |
Veröffentlichung: | 1990 |
Medientyp: | academicJournal |
ISSN: | 0093-7711 (print) |
DOI: | 10.1007/BF00211642 |
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