Simian alphaherpesviruses and their relation to the human herpes simplex viruses.
In: Archives of Virology, Jg. 109 (1989-11-01), Heft 1/2, S. 83-102
Online
academicJournal
Zugriff:
Biochemical and immunological properties of structural and nonstructural polypeptides of the human simplex viruses (HSV 1 and HSV 2) and four related herpesviruses of non-human primates [ Herpesvirus simiae (B virus), H. cercopithicus (SA 8), H. saimiri 1 (HVS 1), and H. ateles 1 (HVA 1)] were compared. Using a radioimmunoassay (RIA), the presence of antigenic determinants shared among all six viruses was demonstrated. The relative degree of antigenic cross-reactivity among these viruses was further assessed by competition RIA. Antigenically, HSV 1 and HSV 2 were most closely related to each other although both SA 8 and B virus were also very closely related to HSV 1. Considerably less cross-reactivity existed between either HVS 1 or HVA 1 and the other four primate herpesviruses. Cross-hybridization between simian and human herpesvirus genomes demonstrated that extensive homology exists between each of the simian viruses and both HSV 1 and HSV 2. Viral polypeptides bearing common antigenic determinants were identified by immune precipitation of infected cell polypeptides and by immunoblotting. Among the polypeptides of HSV which were recognized by antisera to simian viruses were the VP 5 and p40 proteins, both of which are structural components of the virion nucleocapsid. Using recombinant plasmids containing sequences of the HSV 1 VP 5, p 40, DNA polymerase, major DNA binding protein, and TK enzyme genes, homologous sequences were detected in all four simian viruses. Together, these results demonstrate that HSV 1, HSV 2, SA 8, and B virus form a closely related sub-group of the primate herpesviruses; HVS 1 and HVA 1 are also related to the other four primate herpesviruses, albeit more distantly. [ABSTRACT FROM AUTHOR]
Copyright of Archives of Virology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Simian alphaherpesviruses and their relation to the human herpes simplex viruses.
|
---|---|
Autor/in / Beteiligte Person: | Hilliard, Julia ; Black, Darla ; Eberle, R. |
Link: | |
Zeitschrift: | Archives of Virology, Jg. 109 (1989-11-01), Heft 1/2, S. 83-102 |
Veröffentlichung: | 1989 |
Medientyp: | academicJournal |
ISSN: | 0304-8608 (print) |
DOI: | 10.1007/BF01310520 |
Sonstiges: |
|