Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells.
In: Capillary Electrophoresis of Carbohydrates (9781607618744); 2011, p105-126, 22p
Buch
Zugriff:
The surfaces of biofilm-positive microorganisms are usually covered with biofilm-specific extracellular polysaccharide substances that play a key role in a biofilm formation and function [1,2]. The presence of this substance on the surface can affect the physicochemical properties of the bacterial cell, including the cell-surface hydrophobicity and surface charge. The differences in the surface charges lead to the different isoelectric points and the different electromigration characteristics of biofilm-positive and biofilm-negative cells. For this reason, the use of the capillary electrophoresis, especially capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) [3,4], appears to be the appropriate method for the detection of biofilm positivity in a particular strain, as well as for the separation of biofilm-positive and biofilm-negative strains. [ABSTRACT FROM AUTHOR]
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Titel: |
Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells.
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Autor/in / Beteiligte Person: | Ruzicka, Filip ; Horka, Marie ; Hola, Veronika |
Quelle: | Capillary Electrophoresis of Carbohydrates (9781607618744); 2011, p105-126, 22p |
Veröffentlichung: | 2011 |
Medientyp: | Buch |
ISBN: | 978-1-60761-874-4 (print) |
DOI: | 10.1007/978-1-60761-875-1_5 |
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