A novel GTP-binding protein hGBP3 interacts with NIK/HGK
In: FEBS Letters, Jg. 530 (2002-10-23), Heft 1-3, S. 233-238
Online
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Zugriff:
A novel human guanylate-binding protein (GBP) hGBP3 was identified and characterized. Similar as the two human guanylate-binding proteins hGBP1 and hGBP2, hGBP3 has the first two motifs of the three classical guanylate-binding motifs, GXXXXGKS (T) and DXXG, but lacks the N (T) KXD motif. Escherichia coli-expressed hGBP3 protein specifically binds to guanosine triphosphate (GTP). Using a yeast two-hybrid system, it was revealed that the N-terminal region of hGBP3 binds to the C-terminal regulatory domain of NIK/HGK, a member of the group I GCK (germinal center kinase) family. This interaction was confirmed by in vitro glutathione-S-transferase (GST) pull-down and co-immunoprecipitation assays. [Copyright &y& Elsevier]
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Titel: |
A novel GTP-binding protein hGBP3 interacts with NIK/HGK
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Autor/in / Beteiligte Person: | Luan, Zhidong ; Zhang, Yan ; Liu, Aihua ; Man, Yunfang ; Cheng, Lu ; Hu, Gengxi |
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Zeitschrift: | FEBS Letters, Jg. 530 (2002-10-23), Heft 1-3, S. 233-238 |
Veröffentlichung: | 2002 |
Medientyp: | academicJournal |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/S0014-5793(02)03467-1 |
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