Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes.
In: Journal of Indian Society of Periodontology, Jg. 16 (2012-10-01), Heft 4, S. 498-503
Online
academicJournal
Zugriff:
Background: In periodontology, Guided Tissue Regeneration (GTR) is based on the concept of providing a space for entry of cells with regenerative potential into the wound environment to initiate the regeneration of structures lost due to periodontal disease. First generation GTR membranes were primarily non-absorbable membranes like expanded polytetrafluorethylene which required a second surgery for its removal. This led researchers to explore absorbable materials like collagen and synthetic biodegradable polymers to fabricate GTR membranes. In the present study, biodegradable Polylactic acid (PLA) is used to fabricate membranes with the potential to be used for GTR therapy. Materials and Methods: Biocompatibility of the PLA membranes were evaluated in a subcutaneous guinea pig model. Antimicrobial effect of the drug-loaded PLA membranes were assessed against a drug-resistant Staphylococcus aureus bacterial isolate. The cytocompatibility of the drug-loaded membranes were evaluated using HeLa cell lines. Results: The PLA membranes were shown to be biocompatible. The drug-loaded PLA membranes showed significant activity against the bacterial isolate. Among the drug-loaded membranes, tetracycline-loaded membrane showed minimal cellular toxicity. Conclusion: The results of this study indicate that biodegradable drug-releasing polylactide membranes have the potential to be used for periodontal regeneration. It has the necessary characteristics of a GTR membrane like biocompatibility, space maintaining ability, and tissue integration. Among the various antimicrobial agents loaded in the PLA membranes, tetracycline-loaded membranes exhibited minimal cellular toxicity against HeLa cells; at the same time showing significant activity against a pathogenic bacterium. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Indian Society of Periodontology is the property of Wolters Kluwer India Pvt Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes.
|
---|---|
Autor/in / Beteiligte Person: | Thomas, Nebu G. ; Sanil, George P. ; Gopimohan, Rajmohan ; Prabhakaran, Jayachandran V. ; Thomas, George ; Panda, Amulya K. |
Link: | |
Zeitschrift: | Journal of Indian Society of Periodontology, Jg. 16 (2012-10-01), Heft 4, S. 498-503 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0972-124X (print) |
DOI: | 10.4103/0972-124X.106883 |
Sonstiges: |
|