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Using muscle gene expression to estimate triacylglyceride deposition, and relative contributions of fatty acid synthesis and fatty acid import in intramuscular fat in cattle.
In: Animal Production Science, Jg. 54 (2014-09-01), Heft 9, S. 1436-1442
Online
academicJournal
Zugriff:
Intramuscular fat content (IMF%) in cattle influences the value of individual animals, especially for higher marbling markets. IMF is triacylglyceride (TAG) in lipid droplets in the intramuscular adipocytes. However, there are many different pathways from feed intake to the final common process of TAG synthesis and storage as IMF. To evaluate the relative importance of different pathways we compared changes in the expression of genes encoding proteins involved in the TAG and fatty acid (FA) synthesis pathways in the longissimus muscle of Piedmontese x Hereford (PxH) and Wagyu x Hereford (WxH) crosses. Based on these changes we have estimated the relative contributions of FA synthesised de novo in the intramuscular adipocyte and the uptake of circulating FA (both free and from TAG), from the diet or synthesised de novo in other tissues, to TAG deposition as IMF. We have analysed the impact of different developmental times and different diets on these processes. Increased de novo FA synthesis in intramuscular adipocytes appeared to contribute more than increased FA uptake from circulation to the additional TAG deposition in WxH compared with PxH cattle between 12 and 25 months (forage diet). Changing diet from forage to concentrate appeared to increase the importance of FA uptake from circulation relative to de novo FA synthesis for TAG synthesis in intramuscular adipocytes. These results are consistent with the literature based on analysis of lipid composition. Gene expression appears to provide a simple assay for identification of the source of FA for the deposition of IMF. Intramuscular fat in beef is an important factor in its flavour and texture, and hence price. We have used the analysis of the expression of genes in muscle to identify the source of the fat; either synthesised in the muscle fat cells, or taken up from circulation. We have shown that age, diet and breed affect the amount and source of the fat and predict consequential impacts on fat composition. [ABSTRACT FROM AUTHOR]
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Titel: |
Using muscle gene expression to estimate triacylglyceride deposition, and relative contributions of fatty acid synthesis and fatty acid import in intramuscular fat in cattle.
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Autor/in / Beteiligte Person: | Dalrymple, B. P. ; Guo, B. ; Zhou, G. H. ; Zhang, W. |
Zeitschrift: | Animal Production Science, Jg. 54 (2014-09-01), Heft 9, S. 1436-1442 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 1836-0939 (print) |
DOI: | 10.1071/AN14247 |
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