The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice.
In: Pathogens & Disease, Jg. 72 (2014-12-01), Heft 3, S. 188-196
Online
academicJournal
Zugriff:
Lipoproteins are known to be effective immunogens and affect both innate and adaptive immunity. The lprN gene of Mycobacterium tuberculosis has been predicted to encode for a putative lipoprotein in silico. Here, we studied its function as an immunogen by in vivo studies in mice. The recombinant LprN protein, expressed and purified in Escherichia coli, triggered a cell-mediated immune response in BALB/c mice. This was observed by significantly higher T-cell proliferation and increased production of TNF-α and IFN-γ cytokines. However, pre-exposure to LprN protein failed to provide protection in mice after challenge with a virulent strain of M. tuberculosis. Histological examination showed an increase in tissue destruction in experimental animals, indicating an immunogenic potential for LprN protein that enhanced the virulence of bacilli. [ABSTRACT FROM AUTHOR]
Copyright of Pathogens & Disease is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice.
|
---|---|
Autor/in / Beteiligte Person: | Pasricha, Rashmi ; Saini, Neeraj K. ; Rathor, Nisha ; Pathak, Rakesh ; Sinha, Rajesh ; Varma-Basil, Mandira ; Mishra, Kiran ; Brahmachari, Vani ; Bose, Mridula |
Link: | |
Zeitschrift: | Pathogens & Disease, Jg. 72 (2014-12-01), Heft 3, S. 188-196 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 2049-632X (print) |
DOI: | 10.1111/2049-632X.12200 |
Schlagwort: |
|
Sonstiges: |
|