HNE Induces the Hyperexpression of MUC5AC in Chronic Rhinosinusitis With Nasal Polyps by Activating the TRAF6/Autophagy Regulatory Axis.
In: American Journal of Rhinology & Allergy, Jg. 36 (2022-11-01), Heft 6, S. 816-826
academicJournal
Zugriff:
Background: Hypersecretion of mucin 5AC (MUC5AC) is a prominent feature of chronic rhinosinusitis with nasal polyps (CRSwNP) and autophagy plays a pivotal role in this process. TNF receptor-associated factor 6 (TRAF6) functions as a signal transducer in many inflammation diseases, whereas the correlation between TRAF6 and autophagy in CRSwNP remains unclear. Objective: To investigate the role of TRAF6 in the human neutrophil elastase (HNE)-induced autophagy and mucin MUC5AC over-expression in CRSwNP. Methods: Tissue specimens were obtained from control subjects and patients with CRSwNP. The relationships between HNE, TRAF6, autophagy, and MUC5AC were investigated. The effect of TRAF6 on HNE-mediated autophagy and hypersecretion of MUC5AC was assessed by in-vitro culture of HNECs treated with human recombinant HNE. Results: Patients with CRSwNP had more protein expression of HNE, MUC5AC, TRAF6, and light chain (LC3B), and increased levels of Beclin-1(BECN1) and autophagy-related gene 5 (ATG5) in mRNA level. Treatment of nasal epithelial cells with recombinant HNE induced the upregulation of TRAF6, autophagy, and MUC5AC. Alternatively, si-TRAF6 or autophagy inhibitor treatment mitigates the hyperexpression of MUC5AC before incubating with recombinant HNE. Conclusion: HNE promotes autophagy through TRAF6, resulting in hyperexpression of MUC5AC in CRSwNP. [ABSTRACT FROM AUTHOR]
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Titel: |
HNE Induces the Hyperexpression of MUC5AC in Chronic Rhinosinusitis With Nasal Polyps by Activating the TRAF6/Autophagy Regulatory Axis.
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Autor/in / Beteiligte Person: | Zhang, Ying ; Qi, Jing ; Yan, Danqing ; Deng, Yangquan ; Zhang, Jian ; Luo, Qing |
Zeitschrift: | American Journal of Rhinology & Allergy, Jg. 36 (2022-11-01), Heft 6, S. 816-826 |
Veröffentlichung: | 2022 |
Medientyp: | academicJournal |
ISSN: | 1945-8924 (print) |
DOI: | 10.1177/19458924221116939 |
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