Development of a novel fluorogenic assay method for screening inhibitors of bovine leukemia virus protease and identification of mitorubrinic acid as an anti-BLV compound.
In: Bioscience, Biotechnology & Biochemistry, Jg. 87 (2023-09-01), Heft 9, S. 946-953
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Zugriff:
Bovine leukemia virus (BLV) causes enzootic bovine leukosis, a fatal cattle disease that leads to significant economic losses in the livestock industry. Currently, no effective BLV countermeasures exist, except testing and culling. In this study, we developed a high-throughput fluorogenic assay to evaluate the inhibitory activity of various compounds on BLV protease, an essential enzyme for viral replication. The developed assay method was used to screen a chemical library, and mitorubrinic acid was identified as a BLV protease inhibitor that exhibited stronger inhibitory activity than amprenavir. Additionally, the anti-BLV activity of both compounds was evaluated using a cell-based assay, and mitorubrinic acid was found to exhibit inhibitory activity without cytotoxicity. This study presents the first report of a natural inhibitor of BLV protease—mitorubrinic acid—a potential candidate for the development of anti-BLV drugs. The developed method can be used for high-throughput screening of large-scale chemical libraries. This work reports the development of a novel fluorogenic assay method for screening inhibitors of bovine leukemia virus protease. [ABSTRACT FROM AUTHOR]
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Titel: |
Development of a novel fluorogenic assay method for screening inhibitors of bovine leukemia virus protease and identification of mitorubrinic acid as an anti-BLV compound.
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Autor/in / Beteiligte Person: | Murakami, Hironobu ; Fujikawa, Yuuta ; Mori, Masaya ; Mosu, Nozomi ; Taguchi, Akihiro ; Hayashi, Yoshio ; Inoue, Hideshi ; Kamisuki, Shinji |
Zeitschrift: | Bioscience, Biotechnology & Biochemistry, Jg. 87 (2023-09-01), Heft 9, S. 946-953 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 0916-8451 (print) |
DOI: | 10.1093/bbb/zbad073 |
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