Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in.
In: Bioscience, Biotechnology & Biochemistry, Jg. 87 (2023-11-01), Heft 11, S. 1285-1294
academicJournal
Zugriff:
Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium. [ABSTRACT FROM AUTHOR]
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Titel: |
Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in.
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Autor/in / Beteiligte Person: | Watanabe, Yuko ; Katsumura, Eri ; Domon, Tatsuki ; Ishikawa, Yuta ; Oguri, Rina ; Takashima, Minami ; Meng, Qi ; Kinoshita, Masato ; Hashimoto, Hisashi ; Hitomi, Kiyotaka |
Zeitschrift: | Bioscience, Biotechnology & Biochemistry, Jg. 87 (2023-11-01), Heft 11, S. 1285-1294 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 0916-8451 (print) |
DOI: | 10.1093/bbb/zbad116 |
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