Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress.
In: Osteoarthritis & Cartilage, Jg. 22 (2014-06-01), Heft 6, S. 845-851
Online
academicJournal
Zugriff:
Abstract: Objective: Excessive mechanical stress is considered a major cause of temporomandibular joint osteoarthritis (TMJ-OA). High magnitude cyclic tensile strain (CTS) up-regulates pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in chondrocytes, while selective cyclooxygenase (COX)-2 inhibition has been shown to be beneficial to cytokine-induced cartilage damage. However, the effect of selective COX-2 inhibitors on mechanically stimulated chondrocytes remains unclear. This study evaluated the effect of celecoxib, a selective COX-2 inhibitor, on extracellular matrix (ECM) metabolism of mandibular condylar chondrocytes under CTS. Methods: Porcine mandibular chondrocytes were subjected to CTS of 0.5 Hz, 10% elongation with celecoxib for 24 h. The gene expressions of COX-2, MMPs, aggrecanase (ADAMTS), type II collagen and aggrecan were examined by real-time PCR. Also, prostaglandin E 2 (PGE 2 ) concentrations were determined using enzyme immunoassay kit. The levels of MMP and transcription factor NF-κB were measured by western blot while MMP activity was determined by casein zymography. Results: The presence of celecoxib normalized the release of PGE 2 and diminished the CTS-induced COX-2, MMP-1, MMP-3, MMP-9 and ADAMTS-5 gene expressions while recovered the downregulated type II collagen and aggrecan gene expressions. Concurrently, celecoxib showed inhibition of NF-κB and suppression of MMP production and activity. Conclusions: Celecoxib exerts protective effects on mandibular condylar chondrocytes under CTS stimulation by diminishing degradation and restoring synthesis of ECM. [Copyright &y& Elsevier]
Copyright of Osteoarthritis & Cartilage is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress.
|
---|---|
Autor/in / Beteiligte Person: | Su, S. C. ; Tanimoto, K. ; Tanne, Y. ; Kunimatsu, R. ; Hirose, N. ; Mitsuyoshi, T. ; Okamoto, Y. ; Tanne, K. |
Link: | |
Zeitschrift: | Osteoarthritis & Cartilage, Jg. 22 (2014-06-01), Heft 6, S. 845-851 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 1063-4584 (print) |
DOI: | 10.1016/j.joca.2014.03.011 |
Sonstiges: |
|