Enhanced Proteolytic Processing of the Human Immunodeficiency Virus Type 1 Envelope Protein in Murine Ltk(-) Cells
In: AIDS Research and Human Retroviruses, Jg. 11 (1995), Heft 1, S. 81-85
serialPeriodical
Zugriff:
Proteolytic processing of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) precursor glycoprotein (gp 160) to produce the mature gp120 and gp41 proteins is required for virus infection and virus-induced cell fusion. It has also been suggested that cleavage of gp120 at the immunodominant V3 loop region is required for virus-to-cell and cell-to-cell fusion. In this investigation we have studied the proteolytic processing of the HIV-1 Env in cells of various origins (human, monkey, and mouse) infected with a vaccinia virus recombinant expressing the entire gp160 protein (VV-env-1). We have observed that in murine Ltk(-) cells, in addition to the proteolytic cleavage of gp160 at the gp 120/gp41 site, there is also extensive intracellular proteolytic processing of gp160 at the V3 loop and at a novel site located at the C terminus of gp41. Similar proteolytic processing of the Env precursor was observed after treatment of extracts of VV-env-1-infected monkey cells with thrombin, a trypsin-like protease that has been shown to cleave the gp120 at the V3 loop. Our findings suggest that murine Ltk(-) cells could be a good model system for structural studies of Env with different HIV isolates and in searches for proteinase inhibitors that could prevent HIV-1 infection of susceptible cells by blocking proteolysis of Env.
Titel: |
Enhanced Proteolytic Processing of the Human Immunodeficiency Virus Type 1 Envelope Protein in Murine Ltk(-) Cells
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Zeitschrift: | AIDS Research and Human Retroviruses, Jg. 11 (1995), Heft 1, S. 81-85 |
Veröffentlichung: | 1995 |
Medientyp: | serialPeriodical |
ISSN: | 0889-2229 (print) ; 1931-8405 (print) |
DOI: | 10.1089/aid.1995.11.81 |
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