Emergence of a type II collagen-specific helper T cell response
In: European Journal of Immunology, Jg. 31 (2001-08-01), Heft 8, S. 2362-2372
Online
serialPeriodical
Zugriff:
Antigen-driven development of persistent self-reactive Th cells underlies the chronic, progressive nature of many autoimmune diseases. It is crucial to understand the behavior of these self-reactive Th cells; however, they have been notoriously difficult to isolate directly ex vivo. Collagen-induced arthritis (CIA) can be initiated in I-A q -expressing mice (DBA/1) using heterologous type II collagen (cII) immunization and is dependent on Th cells that are specific for a single immunodominant epitope. Here, we identify one compartment of cII-specific Th cell using TCRβ expression, cell surface phenotype, and direct single-cell repertoire analysis. A subpopulation of CD4 + Vβ10 + T cells up-regulates both CD44 and GL7 and expands significantly in response to initial priming in the majority of animals (D9: 70%). The cII-specific Vβ10 + primary responders are further resolved through expression of a highly restricted junctional region, previously associated with autoimmune disease. This cII-specific clonotype rapidly re-expands upon antigen recall and can be isolated from the lymph nodes of arthritic animals. These single-cell analyses quantify the emergence, decline and rapid re-emergence of a self-reactive Th cell population in vivo and outline one strategy for isolating these cells directly ex vivo.
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Emergence of a type II collagen-specific helper T cell response
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Autor/in / Beteiligte Person: | Pogue-Caley, Rebecca R. ; McHeyzer-Williams, Michael G. |
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Zeitschrift: | European Journal of Immunology, Jg. 31 (2001-08-01), Heft 8, S. 2362-2372 |
Veröffentlichung: | 2001 |
Medientyp: | serialPeriodical |
ISSN: | 0014-2980 (print) ; 1521-4141 (print) |
DOI: | 10.1002/1521-4141(200108)31:8<2362::AID-IMMU2362>3.0.CO;2-O |
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