Human T cell recognition of the Mycobacterium lepraeLSR antigen: epitopes and HLA restriction
In: FEMS Immunology and Medical Microbiology, Jg. 24 (1999-06-01), Heft 2, S. 151-159
Online
serialPeriodical
Zugriff:
We have in this work mapped epitopes and HLA molecules used in human T cell recognition of the Mycobacterium lepraeLSR protein antigen. HLA typed healthy subjects immunized with heat killed M. lepraewere used as donors to establish antigen reactive CD4+ T cell lines which were screened for proliferative responses against overlapping synthetic peptides covering the C‐terminal part of the antigen sequence. By using this approach we were able to identify two epitope regions represented by peptide 2 (aa 29–40) and peptide 6 (aa 49–60), of which the former was mapped in detail by defining the N‐ and C‐terminal amino acid positions necessary for T cell recognition of the core epitope. MHC restriction analysis showed that peptide 2 was presented to T cells by allogeneic cells coexpressing HLA‐DR4 and DRw53 or DR7 and DRw53. In contrast, peptide 6 was presented to T cells only in the context of HLA‐DR5 molecules. In conclusion, the M. lepraeLSR protein antigen can be recognized by human T cells in the context of multiple HLA‐DR molecules, of which none are reported to be associated with the susceptibility to develop leprosy. The results obtained are in support of using the LSR antigen in subunit vaccine design.
Titel: |
Human T cell recognition of the Mycobacterium lepraeLSR antigen: epitopes and HLA restriction
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Autor/in / Beteiligte Person: | Oftung, Fredrik ; Lundin, Knut E.A. ; Meloen, Robert ; Mustafa, Abu Salim |
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Zeitschrift: | FEMS Immunology and Medical Microbiology, Jg. 24 (1999-06-01), Heft 2, S. 151-159 |
Veröffentlichung: | 1999 |
Medientyp: | serialPeriodical |
ISSN: | 0928-8244 (print) ; 1574-695X (print) |
DOI: | 10.1111/j.1574-695X.1999.tb01276.x |
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