Novel Dictyosteliumunconventional myosin, MyoM, has a putative RhoGEF domain
In: FEBS Letters, Jg. 474 (2000), Heft 1, S. 16-22
Online
serialPeriodical
Zugriff:
We have cloned a novel unconventional myosin gene myoMin Dictyostelium. Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily. Following the motor domain, two calmodulin-binding IQ motifs, a putative coiled-coil region, and a Pro, Ser and Thr-rich domain, lies a combination of dbl homology and pleckstrin homology domains. These are conserved in Rho GDP/GTP exchange factors (RhoGEFs). We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyosteliumcells were not affected by the myoM−mutation. Green fluorescent protein-tagged MyoM, however, accumulated at crown-shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis.
Titel: |
Novel Dictyosteliumunconventional myosin, MyoM, has a putative RhoGEF domain
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Autor/in / Beteiligte Person: | Oishi, Noboru ; Adachi, Hiroyuki ; Sutoh, Kazuo |
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Zeitschrift: | FEBS Letters, Jg. 474 (2000), Heft 1, S. 16-22 |
Veröffentlichung: | 2000 |
Medientyp: | serialPeriodical |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/S0014-5793(00)01564-7 |
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