Genetic Variation of EPAS1Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in China
In: Journal of Integrative Agriculture, Jg. 13 (2014-09-01), Heft 9, S. 1990-1998
Online
serialPeriodical
Zugriff:
Endothelial PAS domain protein 1 (EPAS1), also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this study, polymorphisms of EPAS1were detected in 217 individuals from 2 Tibetan pig populations and 3 low-altitude pig breeds by DNA pooling, PCR-SSCP, PCR-RFLP and DNA sequencing methods. A total of 14 synonymous polymorphisms were identified in the coding region. The analysis suggested that SNP1 (G963A), SNP7 (C1632T), SNP10 (G1929A) and SNP11 (G1947A) showed potential association with high-altitude environment because of their particular variation patterns in Tibetan pigs. Linkage disequilibrium (LD) of these SNPs was analyzed. One common LD block including 5 SNPs clustering in exon 12 was identified in all studied pig populations. Haplotype H1 (AGGTC) in LD block was dominant in Tibetan pigs (76.6 and 74.2% in Linzhi (LZ) and Chayu (CY) pigs, respectively) and segregated at higher frequency than that in low-altitude pig breeds (52.3, 58.7 and 56.2% in Wuzhishan (WZS), Min (M) and Laiwu (LW) pigs, respectively), indicating that H1 may relate to adaptation to high altitude in Tibetan pigs. These findings raise hope that EPAS1gene can be a candidate gene that involved in adaptation of high altitude in Tibetan pigs.
Titel: |
Genetic Variation of EPAS1Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in China
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Autor/in / Beteiligte Person: | DONG, Kun-zhe ; KANG, Ye ; YAO, Na ; SHU, Guo-tao ; ZUO, Qing-qing ; ZHAO, Qian-jun ; MA, Yue-hui |
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Zeitschrift: | Journal of Integrative Agriculture, Jg. 13 (2014-09-01), Heft 9, S. 1990-1998 |
Veröffentlichung: | 2014 |
Medientyp: | serialPeriodical |
ISSN: | 2095-3119 (print) |
DOI: | 10.1016/S2095-3119(13)60577-8 |
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