Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a Dbl family member
In: FEBS Letters, Jg. 459 (1999-10-15), Heft 3, S. 313-318
Online
serialPeriodical
Zugriff:
Several guanine nucleotide exchange factors (GEFs) for Rho-GTPases have been identified, all of them containing a Dbl homology (DH) and pleckstrin homology (PH) domain, but exhibiting different specificities to the Rho family members, Rho, Rac and Cdc42. We report here that KIAA0380, a protein with a tandem DH/PH domain, an amino-terminal PDZ domain and a regulator of G protein signalling (RGS) homology domain, is a specific GEF for RhoA, but not for Rac1 and Cdc42, as determined by GDP release, guanosine 5′- O -(3-thio)triphosphate (GTPγS) binding and protein binding assays. When expressed in J82 cells, DH/PH domain-containing forms of KIAA0380 induced actin stress fibers, whereas expression of the RGS homology domain prevented lysophosphatidic acid (LPA)-induced stress fiber formation.
Titel: |
Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a Dbl family member
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Autor/in / Beteiligte Person: | Ru¨menapp, Ulrich ; Blomquist, Andrea ; Schwo¨rer, Guntram ; Schablowski, Helge ; Psoma, Amalia ; Jakobs, Karl H. |
Link: | |
Zeitschrift: | FEBS Letters, Jg. 459 (1999-10-15), Heft 3, S. 313-318 |
Veröffentlichung: | 1999 |
Medientyp: | serialPeriodical |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/S0014-5793(99)01270-3 |
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