Enzymatic assay for cholesterol ester hydrolase activity.
In: Journal of Lipid Research, Jg. 19 (1978-09-01), Heft 7, S. 913-916
Online
serialPeriodical
Zugriff:
A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol oxidase and peroxidase. The free cholesterol remaining after the cholesterol ester hydrolase-catalyzed esterification is converted to delta 4-cholestenone and hydrogen peroxide (H2O2); peroxidase couples H2O2 with phenol and 4-amino-antipyrine to yield a stable rose-colored product absorbing at 500 nm. The method is highly reproducible and the values correlate well with those obtained with the chromatographic radioassay of CEH activity.
Titel: |
Enzymatic assay for cholesterol ester hydrolase activity.
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Autor/in / Beteiligte Person: | Gallo, L L ; Atasoy, R ; Vahouny, G V ; Treadwell, C R |
Link: | |
Zeitschrift: | Journal of Lipid Research, Jg. 19 (1978-09-01), Heft 7, S. 913-916 |
Veröffentlichung: | 1978 |
Medientyp: | serialPeriodical |
ISSN: | 0022-2275 (print) ; 1539-7262 (print) |
DOI: | 10.1016/S0022-2275(20)40706-0 |
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