The Human Chemoattractant Complement C5a Receptor Inhibits Cyclic AMP Accumulation Through G<SUB>i</SUB> and G<SUB>z</SUB> Proteins
In: Biochemical and Biophysical Research Communications, Jg. 208 (1995-03-08), Heft 1, S. 223-229
serialPeriodical
Zugriff:
The human C5a receptor is known to signal through G i proteins. The ability of the cloned C5a receptor to inhibit adenylyl cyclase or to stimulate phospholipase C through G i proteins was examined in transfected cells. Activation of recombinant C5a receptors resulted in the stimulation of phospholipase C in Ltk − cells and inhibition of adenylyl cyclase in 293 cells. Pertussis toxin potently abolished both responses indicating the involvement of G i proteins. Previous studies have shown that G i -mediated inhibition of adenylyl cyclase can be similarly regulated by the pertussis toxin-insensitive G z . In 293 cells co-transfected with the α subunit of G z , the C5a-mediated inhibition of cAMP accumulation became pertussis toxin-resistant, signifying functional coupling between the C5a receptor and G z . However, G z cannot substitute for G i in the C5a-induced stimulation of phospholipase C or inhibition of adenylyl cyclase in Ltk − cells.Copyright 1995, 1999 Academic Press, Inc.
Titel: |
The Human Chemoattractant Complement C5a Receptor Inhibits Cyclic AMP Accumulation Through G<SUB>i</SUB> and G<SUB>z</SUB> Proteins
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Autor/in / Beteiligte Person: | Shum, J.K. ; Allen, R.A. ; Wong, Y.H. |
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Zeitschrift: | Biochemical and Biophysical Research Communications, Jg. 208 (1995-03-08), Heft 1, S. 223-229 |
Veröffentlichung: | 1995 |
Medientyp: | serialPeriodical |
ISSN: | 0006-291X (print) ; 1090-2104 (print) |
DOI: | 10.1006/bbrc.1995.1327 |
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