Theophyllin induziert Kontrakturen an Skelettmuskelpräparaten des Schweines mit Disposition zu Maligner Hyperthermie
In: ains · Anästhesiologie · Intensivmedizin · Notfallmedizin · Schmerztherapie, Jg. 39 (2004-03-01), S. 147-152
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Zugriff:
Objective: Theophylline, a methylxanthine, leads to an increase of the cytoplasmic Ca 2 + -concentration in the muscle cell. Since the in-vitro contracture test (IVCT) with halothane and caffeine does not distinguish a 100% between malignant hyperthermia susceptible (MHS) and non-susceptible (MHN), we examined the in-vitro effects of theophylline in porcine skeletal muscle preparations. Methods: After approval by the local animal care committee ten MHS- and nine MHN-swine were anaesthetized and muscle biopsies taken. For IVCT, muscle specimens were exposed to bolus administrations of theophylline in concentrations of 3.0 respectively 5.0 mmol/l. Muscle contracture development and twitch amplitudes were recorded over a period of 30 minutes. Data are expressed as medians and ranges. Results: After both theophylline bolus administrations MHS-muscles developed significantly higher contractures compared to the MHN-specimens. The MHS-muscles reached a maximum contracture of 17.0mN (7.2-59.6mN) after administration of 3.0mmol/l theophylline. In comparison, two MHN-specimens showed weak contractures with a maximum of 1.4 mN. The 5.0 mmol/l theophylline IVCT resulted in maximum contractures of 19.1 mN (2.1-39.2mN) for the MHS-preparations. Just in three MHN-muscles weak contractures of 0.0mN (0.0 - 0.8 mN) were recorded. Thus, a significant difference without overlap was revealed for the maximum contracture. Conclusion: Theophylline in concentrations of 3.0 and 5.0 mmol/l revealed a clear difference between MHS- and MHN-porcine muscle preparations. Further examinations on human skeletal muscles are needed to demonstrate the value of theophylline in the IVCT MH-diagnosis.
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Theophyllin induziert Kontrakturen an Skelettmuskelpräparaten des Schweines mit Disposition zu Maligner Hyperthermie
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Autor/in / Beteiligte Person: | Weisshorn, Ralf ; Wappler, Frank ; Gerbershagen, M. U. ; Fiege, Marko ; Kolodzie, Kerstin |
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Zeitschrift: | ains · Anästhesiologie · Intensivmedizin · Notfallmedizin · Schmerztherapie, Jg. 39 (2004-03-01), S. 147-152 |
Veröffentlichung: | Georg Thieme Verlag KG, 2004 |
Medientyp: | unknown |
ISSN: | 1439-1074 (print) ; 0939-2661 (print) |
DOI: | 10.1055/s-2004-814328 |
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